D283 Med (ATCC® HTB-185)

Organism: Homo sapiens, human  /  Cell Type: Medulloblastoma  /  Tissue: brain/cerebellum; derived from metastatic site: peritoneum  /  Disease: medulloblastoma

Organism Homo sapiens, human
Tissue
brain/cerebellum; derived from metastatic site: peritoneum
Cell Type Medulloblastoma
Product Format frozen
Morphology epithelial
Culture Properties suspension, multicell aggregates and some adherent cells
Biosafety Level 1
Disease medulloblastoma
Age 6 years juvenile
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype The karyotype is 45, XY, -7, -8, -17, -20, der(20)t(1;20)(q12;q13), 8q+, 17p+ (range = 41 to 46). This is a hypodiploid cell line with a frequency of higher ploidies of 5.4%. Three marker chromosomes are present in all cells. They are: der(20)t(1;20)(q12;q13), 8q+ and 17p+. N7, N17 and N20 have single copies. The single X is structurally normal, and the Y chromosome is present as confirmed by fluorescence microscopy.
Derivation
The D283 Med cell line was established in 1985 by Friedman et al. from malignant ascites cells and a peritoneal metastasis from a boy with medulloblastoma. ­
Derived from metastatic site, peritoneum
Clinical Data
6 years juvenile
Caucasian
The D283 Med cell line was established in 1985 by Friedman et al. from malignant ascites cells and a peritoneal metastasis from a boy with medulloblastoma.
male
Genes Expressed
The cells produce tumors in nude mice, and the resulting tumors are positive for expression of neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein.
The cells express elevated levels of four biochemical markers of SCLC (neuron specific enolase, the brain isoenzyme of creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity.
Cellular Products
neurofilament proteins (NFP)
Tumorigenic Yes
Effects
Yes, in nude mice
Yes, in soft agar
Comments
The cells produce tumors in nude mice, and the resulting tumors are positive for expression of neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of medium. Adherent cells can be dislodged by scraping, and cultures can be established by resuspending a cell pellet at 5 X 105viable cells/mL. Maintain cultures between 4 X 105 and 1 X 105 cells/mL.
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: culture medium, 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X,Y
CSF1PO: 9,12
D13S317: 8,10
D16S539: 11
D5S818: 11
D7S820: 10
THO1: 7
TPOX: 8,11
vWA: 16,18
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 0
PGM1, 1
PGM3, 1
Population Doubling Time 52 hrs
Name of Depositor HS Friedman
Year of Origin 1985
References

Friedman HS, et al. Phenotypic and genotypic analysis of a human meduloblastoma cell line and transplantable xenograft (D341 Med) demonstrating amplification of c-myc. Am. J. Pathol. 130: 472-484, 1988. PubMed: 3279793

He XM, et al. Expression of O6-methylguanine-DNA methyltransferase in six human medulloblastoma cell lines. Cancer Res. 52: 1144-1148, 1992. PubMed: 1737373

Friedman HS, et al. Establishment and characterization of the human medulloblastoma cell line and transplantable xenograft D283 Med. J. Neuropathol. Exp. Neurol. 44: 592-605, 1985. PubMed: 4056828

Trojanowski JQ, et al. A rapidly dividing human medulloblastoma cell line (D283 MED) expresses all three neurofilament subunits. Am. J. Pathol. 126: 358-363, 1987. PubMed: 3103453

Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024

The cells form serially transplantable intercranial and subcutaneous tumors.

Basic Documentation
References

Friedman HS, et al. Phenotypic and genotypic analysis of a human meduloblastoma cell line and transplantable xenograft (D341 Med) demonstrating amplification of c-myc. Am. J. Pathol. 130: 472-484, 1988. PubMed: 3279793

He XM, et al. Expression of O6-methylguanine-DNA methyltransferase in six human medulloblastoma cell lines. Cancer Res. 52: 1144-1148, 1992. PubMed: 1737373

Friedman HS, et al. Establishment and characterization of the human medulloblastoma cell line and transplantable xenograft D283 Med. J. Neuropathol. Exp. Neurol. 44: 592-605, 1985. PubMed: 4056828

Trojanowski JQ, et al. A rapidly dividing human medulloblastoma cell line (D283 MED) expresses all three neurofilament subunits. Am. J. Pathol. 126: 358-363, 1987. PubMed: 3103453

Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024

The cells form serially transplantable intercranial and subcutaneous tumors.