NG108-15 [108CC15] (ATCC® HB-12317)

Organism: Mus musculus (neuroblastoma); Rattus norvegicus (glioma), mouse (neuroblastoma); rat (glioma)  /  Cell Type: somatic cell hybrid  /  Tissue: brain  /  Disease: glioblastoma; neuroblastoma

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Organism Mus musculus (neuroblastoma); Rattus norvegicus (glioma), mouse (neuroblastoma); rat (glioma)
Tissue
brain
Cell Type somatic cell hybrid
Product Format frozen
Morphology flat; round; 10 to 100 micrometers diameter
Culture Properties Adherent, but please note: as the culture media becomes acidic these cells begin to detach and grow as a suspension, but they will typically reattach again when fresh medium is added.
Biosafety Level 1
Disease glioblastoma; neuroblastoma
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
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Derivation
The line was formed by fusing mouse N18TG2 neuroblastoma cells with rat C6-BU-1 glioma cells in the presence of inactivated Sendai virus.
The NG108-15 cell line, originally named 108CC15, was developed in 1971 by Bernd Hamprecht.
Complete Growth Medium The base medium for this cell line is Dulbecco's Modified Eagle's Medium (GIBCO/InVitrogen Catalog No.12100-061, DMEM without sodium pyruvate ). To make the complete growth medium, add the following components to the base medium:
  • 0.1 mM hypoxanthine (final conc.)
  • 400 nM aminopterin (final conc.)
  • 0.016 mM thymidine (final conc.)
  • 10% fetal bovine serum (final conc.)
  • 1.5 g/L sodium bicarbonate

Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium, 92.5%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Name of Depositor Univ. Texas Southwestern Medical Cntr.
References

Hamprecht B. Structural, electrophysiological, biochemical, and pharmacological properties of neuroblastoma-glioma cell hybrids in cell culture. Int. Rev. Cytol. 49: 99-170, 1977. PubMed: 16829

Hamprecht B, et al. Culture and characteristics of hormone-responsive neuroblastoma X glioma hybrid cells. Methods Enzymol. 109: 316-341, 1985. PubMed: 2985920

Bernd Hamprecht, personal communication

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Hamprecht B. Structural, electrophysiological, biochemical, and pharmacological properties of neuroblastoma-glioma cell hybrids in cell culture. Int. Rev. Cytol. 49: 99-170, 1977. PubMed: 16829

Hamprecht B, et al. Culture and characteristics of hormone-responsive neuroblastoma X glioma hybrid cells. Methods Enzymol. 109: 316-341, 1985. PubMed: 2985920

Bernd Hamprecht, personal communication