SVG p12 (ATCC® CRL-8621)

Organism: Homo sapiens, human  /  Cell Type: astroglia; SV40 transforme  /  Tissue: brain  / 

Organism Homo sapiens, human
Tissue brain
Cell Type astroglia; SV40 transforme
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 2  [Cells contain polyomavirus DNA sequences]
Age fetus, first trimester
Applications
The are susceptible to infection by JC virus, and may be useful in, detecting and cultivating other human neurotropic viruses.

Storage Conditions liquid nirogen vapor phase
Derivation
The SVG p12 cell line was established by transfecting cultured human fetal glial cells from brain material dissected from 8 to 12 week old embryos with DNA from an ori - mutant of SV40.
Genes Expressed
SV40 T protein; glial fibrillary acidic protein (GFAP)
Virus Susceptibility JC polyomavirus
Comments
The cells express SV40 T antigen.

Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels. Seed new flasks (75 cm2) with 5 X 105 cells.
  6. Incubate cultures at 37°C.
    Medium Renewal: 2 to 3 times per week
    Cryopreservation
    Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
    Storage temperature: liquid nirogen vapor phase
    Culture Conditions
    Temperature: 37°C
    Name of Depositor The United States of America
    References

    Major EO. Immortal line of human fetal glial cells. US Patent 4,707,448 dated Nov 17 1987

    Basic Documentation
    References

    Major EO. Immortal line of human fetal glial cells. US Patent 4,707,448 dated Nov 17 1987