CHLA-02-ATRT (ATCC® CRL-3020)

Organism: Homo sapiens, human  /  Tissue: brain/brain tumor  /  Disease: Atypical Teratoid Rhabdoid Tumor (ATRT)

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Organism Homo sapiens, human
Tissue brain/brain tumor
Product Format frozen
Morphology rounded
Culture Properties single cells and clusters in suspension
Biosafety Level 1
Disease Atypical Teratoid Rhabdoid Tumor (ATRT)
Age 1.7 years
Gender male
Storage Conditions liquid nitrogen vapor phase
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Derivation
CHLA-02-ATRT was established from a 1 year old boy with atypical teratoid rhabdoid tumor (ATRT). Cells were isolated from the resected brain tumor at the time of diagnosis and were cultured in neurobasal medium after mechanical disruption.
Comments

Diagnosis of ATRT was validated by immunohistochemistry that demonstrated negative expression of INI-1 in the tumor cells, but normal expression by endothelium on the same section. 

This cell line has been continuously carried in culture for over 18 months, and in neurobasal medium grows as spheres of varying sizes. 

The generation of this cell line was made possible with the support of Grayson's Fund and the Michael Hoefflin Foundation for Children's Cancer to Childrens Hospital Los Angeles, with the goal of making pediatric brain tumor lines available to the research community.

Complete Growth Medium DMEM:F12 Medium (ATCC 30-2006) with 20 ng/mL human recombinant EGF, 20 ng/mL human recombinant basic FGF, and B-27 Supplement (Invitrogen, Cat. No.17504) to a final concentration of 2% (v/v)
Subculturing

Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in ¼ volume of the conditioned medium and ¾ volume fresh medium at 2 X 105 to 3 X 105 viable cells/mL. Do not allow the cell concentration to exceed 2 X 106 cells/mL.

Subcultivation ratio: A subcultivation ratio of 1:4 to 1:6 is recommended.

Medium renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Cryopreservation
Freeze medium: fetal bovine serum, 50%; Cell Freezing Medium-DMSO (Sigma, Cat. No. C-6295), 50%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: XY
CSF1PO: 11,10
D13S317: 11,13
D16S539: 13
D5S818: 11,12
D7S820: 10,12
THO1: 6
TPOX: 10,12
vWA: 16,18
Name of Depositor Anat Erdreich-Epstein, Saban Research institute, Childrens Hospital Los Angeles
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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