EOC 2 (ATCC® CRL-2467)

Organism: Mus musculus, mouse  /  Cell Type: microglia  /  Tissue: brain  / 

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Organism Mus musculus, mouse
Tissue
brain
Cell Type microglia
Product Format frozen
Morphology macrophage
Culture Properties adherent
Biosafety Level 1
Age 10 days
Gender female
Strain C3H/HeJ
Applications
The cells may be used to characterize the role of brain macrophages.
Storage Conditions liquid nitrogen vapor phase
Images
Derivation
This is an immortalized cell line derived from the brain of an apparently normal 10 day old mouse.
Cells were cloned in soft agar in the presence of CSF-1 and expanded on microcarrier beads. Beads were transferred to culture dishes and were subsequently passaged by scraping.
Clinical Data
female
10 days
Antigen Expression
CD11b/CD18 (Mac-1) +, Mac-2 +, Mac-3 +, CD80 (B7-1) +, CD86 (B7.2) +; CD45 +, Ly-6C +, F4/80 +, MHC Class I +, MHC Class II +, CD115 (colony stimulating factor 1 receptor (CSF-1R)) +, FcR +
Receptor Expression
colony stimulating factor 1 (CSF-1R, CD115)
Genes Expressed
CD11b/CD18 (Mac-1) +, Mac-2 +, Mac-3 +, CD80 (B7-1) +, CD86 (B7.2) +; CD45 +, Ly-6C +, F4/80 +, MHC Class I +, MHC Class II +, CD115 (colony stimulating factor 1 receptor (CSF-1R)) +, FcR +
Comments
The cell line is dependent on colony stimulating factor 1 (CSF-1).
The cells exhibit phagocytic activity.

Unlike EOC 13.31 (ATCC CRL-2468) and EOC 20 (ATCC CRL-2469), these cells do not constitutively express high levels of major histocompatibility complex (MHC) class II antigens and expression is not upregulated by recombinant murine interferon-gamma.

C3H/HeJ strain is defective in TLR4 (toll-like receptor 4)

Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 70%; fetal bovine serum, 10%; LADMAC Conditioned Media (produced from the LADMAC cell line (CRL-2420), 20%
Subculturing
  1. Remove and discard 75% of the media.
  2. Scrape off the attached cells with a cell scraper.
  3. Add appropriate aliquots of cell suspension to new culture vessels.
  4. Incubate cultures at 37°C.

    Subcultivation Ratio: 1:2 to 1:4
    Medium Renewal: Every 2 to 3 days
    Cryopreservation
    Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
    Storage temperature: liquid nitrogen vapor phase
    Culture Conditions
    Temperature: 37°C
    Name of Depositor WS Walker
    References

    Olivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247

    Walker WS. Establishment of mononuclear phagocyte cell lines. J. Immunol. Methods 174: 25-31, 1994. PubMed: 8083530

    Walker WS, et al. Mouse microglial cell lines differing in constitutive and interferon-gamma-inducible antigen-presenting activities for naive and memory CD4+ and CD8+ T cells. J. Neuroimmunol. 63: 163-174, 1995. PubMed: 8550814

    Askew D, Walker WS. Alloantigen presentation to naive CD8+ T cells by mouse microglia: evidence for a distinct phenotype based on expression of surface-associated and soluble costimulatory molecules. Glia 18: 118-128, 1996. PubMed: 8913775

    Notice: Necessary PermitsPermits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation
    Other Documentation
    References

    Olivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247

    Walker WS. Establishment of mononuclear phagocyte cell lines. J. Immunol. Methods 174: 25-31, 1994. PubMed: 8083530

    Walker WS, et al. Mouse microglial cell lines differing in constitutive and interferon-gamma-inducible antigen-presenting activities for naive and memory CD4+ and CD8+ T cells. J. Neuroimmunol. 63: 163-174, 1995. PubMed: 8550814

    Askew D, Walker WS. Alloantigen presentation to naive CD8+ T cells by mouse microglia: evidence for a distinct phenotype based on expression of surface-associated and soluble costimulatory molecules. Glia 18: 118-128, 1996. PubMed: 8913775