MC-IXC (ATCC® CRL-2270)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  /  Tissue: brain; derived from metastatic site: supra-orbital area  /  Disease: neuroblastoma

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Organism Homo sapiens, human
Tissue
brain; derived from metastatic site: supra-orbital area
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease neuroblastoma
Age 14 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype doubles minutes (DM) are prevalent
Derivation
MC-IXC is a twice cloned subline of the neuroepithelioma cell line SK-N-MC (see ATCC HTB-10) which was established in September of 1971 from a metastatic tumor mass.
Clinical Data
14 years
Caucasian
female
Antigen Expression
Blood Type O; Rh+
Comments

MC-IXC is a twice cloned subline of the neuroepithelioma cell line SK-N-MC (see ATCC HTB-10) which was established in September of 1971 from a metastatic tumor mass.

The choline acetyltransferase activity of MC-IXC was approximately four times higher than the parental line but MC-IXC was negative for dopamine beta hydroxylase activity as was the parental line.

MC-IXC cells have a reported saturation density greater than 1 X 106 cells/cm2.

The cells are fibroblast-like and grow to form a dense monolayer.

Floating cells are nonviable.
Complete Growth Medium The base medium for this cell line is a 1:1 mixture of ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C

Subculture Ratio: 1:10 to 1:20
Medium Renewal: Every 4 to 7 days.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005.

Cryopreservation
Culture medium, 95%; DMSO, 5%
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 12
D5S818: 11
D7S820: 8
THO1: 9.3
TPOX: 9,11
vWA: 17,18
Population Doubling Time 36 hrs
Name of Depositor JL Biedler
Year of Origin 1971
References

Biedler JL, et al. Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells in continuous culture. Cancer Res. 33: 2643-2652, 1973. PubMed: 4748425

Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. PubMed: 29704

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the cells subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with the Office of Technology Development, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065. Contact Tingting Zhang-Kharas, Direct Phone: 646-888-1083, Reception: 646-888-1080, Email: zhangkht@mskcc.org

References

Biedler JL, et al. Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells in continuous culture. Cancer Res. 33: 2643-2652, 1973. PubMed: 4748425

Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. PubMed: 29704