C6/LacZ (ATCC® CRL-2199)

Organism: Rattus norvegicus, rat  /  Cell Type: glial cell  /  Disease: glioma

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Organism Rattus norvegicus, rat
Cell Type glial cell
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease glioma
Strain outbred
Applications
The cells were cultured in G418 for 14 days, cloned, and evaluated for beta-gal production.
The cells constitutively express the lacZ reporter gene product, E. coli derived beta-gal, as revealed on tissue sections by histochemical stain, and single tumor cells can be identified.
Lymphocytes and other responding cells can be identified by double labeling with antibodies on the same slide.
The contrast between stained cells and background facilitates image analysis.
This is one of few models that permit quantitative analysis of microscopic tumor in the brain.
C6/lacZ, but not 9L/lacZ, shows infiltrative growth in the brain.
The beta-gal expression is less stable than for 9L/lacZ, and the cells should be used soon after thawing or re-cloned.
Derivation
The C6/lacZ cell line was developed in 1989 from the C6 cell line (N-nitrosomethylurea induced rat glial tumor).
The cells constitutively express the lacZ reporter gene product, E. coli derived beta-gal, as revealed on tissue sections by histochemical stain, and single tumor cells can be identified.
Genes Expressed
beta galactosidase (beta-gal)
Cellular Products
beta galactosidase (beta-gal)
Tumorigenic Yes
Effects
Yes, forms tumors in the brains of CD Fischer 344 rats
Comments
The C6/lacZ cell line was developed in 1989 from the C6 cell line (N-nitrosomethylurea induced rat glial tumor).
C6 cells were infected with the BAG replication deficient retroviral vector carrying the E. coli lacZ gene encoding beta-gal and the Tn5 neomycin gene, which confers resistance to G418.
The cells were cultured in G418 for 14 days, cloned, and evaluated for beta-gal production.
The cells constitutively express the lacZ reporter gene product, E. coli derived beta-gal, as revealed on tissue sections by histochemical stain, and single tumor cells can be identified.
Lymphocytes and other responding cells can be identified by double labeling with antibodies on the same slide.
The contrast between stained cells and background facilitates image analysis.
This is one of few models that permit quantitative analysis of microscopic tumor in the brain.
The tumor mimics important features of human brain tumor growth and spread.
The growth pattern of 6C/lacZ complements that of 9L/lacZ (see ATCC CRL-2200). C6/lacZ, but not 9L/lacZ, shows infiltrative growth in the brain.
The beta-gal expression is less stable than for 9L/lacZ, and the cells should be used soon after thawing or re-cloned.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, and 1 mM sodium pyruvate 90%; fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Remove spent medium, rinse with PBS and add fresh 0.25% trypsin, 0.03% EDTA solution and incubate at room temperature (or 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Name of Depositor LA Lampson
References

Lampson LA, et al. Exploiting the lacZ reporter gene for quantitative analysis of disseminated tumor growth within the brain: use of the lacZ gene product as a tumor antigen, for evaluation of antigenic modulation, and to facilitate image analysis of tumor growth in situ. Cancer Res. 53: 176-182, 1993. PubMed: 8416743

Lampson LA, et al. Disseminating tumor cells and their interactions with leukocytes visualized in the brain. Cancer Res. 52: 1018-1025, 1992. PubMed: 1737331

Lampson LA, et al. A new model permits study of the immune response to individual tumor cells within the brain. Neurology 40: 396-397, 1990.

Dutta T, et al. Robust ability of IFN-gamma to upregulate class II MHC antigen expression in tumor bearing rat brains. J. Neuro-Oncol. 64: 31-44, 2003. PubMed: 12952284

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Lampson LA, et al. Exploiting the lacZ reporter gene for quantitative analysis of disseminated tumor growth within the brain: use of the lacZ gene product as a tumor antigen, for evaluation of antigenic modulation, and to facilitate image analysis of tumor growth in situ. Cancer Res. 53: 176-182, 1993. PubMed: 8416743

Lampson LA, et al. Disseminating tumor cells and their interactions with leukocytes visualized in the brain. Cancer Res. 52: 1018-1025, 1992. PubMed: 1737331

Lampson LA, et al. A new model permits study of the immune response to individual tumor cells within the brain. Neurology 40: 396-397, 1990.

Dutta T, et al. Robust ability of IFN-gamma to upregulate class II MHC antigen expression in tumor bearing rat brains. J. Neuro-Oncol. 64: 31-44, 2003. PubMed: 12952284