G355-5 (ATCC® CRL-2033)

Organism: Felis catus, cat  /  Cell Type: astrocyte  / 

Organism Felis catus, cat
Cell Type astrocyte
Product Format frozen
Morphology glial, astrocyte
Culture Properties adherent
Biosafety Level 1
Age embryo
Applications
This is a cloned derivative of the G355 cell line originally produced and distributed by the Naval Biosciences Laboratory.
The line is useful for generating stocks of various C type retroviruses.
Derivation
This is a cloned derivative of the G355 cell line originally produced and distributed by the Naval Biosciences Laboratory.
Comments
This is a cloned derivative of the G355 cell line originally produced and distributed by the Naval Biosciences Laboratory.
The line is useful for generating stocks of various C type retroviruses.
Complete Growth Medium The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium:
  • heat-inactivated fetal bovine serum to a final concentration of 10%
  • Subculturing
    Subcultivation Ratio: A subcultivation ratio of 1:5 is recommended
    Medium Renewal: 2 to 3 times per week
    Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.03% EDTA solution.
    Remove the trypsin, add fresh trypsin (1 to 2 ml) and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes).
    Add fresh medium, aspirate and dispense into new flasks.
    Culture Conditions
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Name of Depositor KJ Dunn
    References

    Haapala DK, et al. Isolation from cats of an endogenous type C virus with a novel envelope glycoprotein. J. Virol. 53: 827-833, 1985. PubMed: 2983093

    Bassin RH, et al. Normal DBA/2 mouse cells synthesize a glycoprotein which interferes with MCF virus infection. Virology 123: 139-151, 1982. PubMed: 6959413

    Basic Documentation
    References

    Haapala DK, et al. Isolation from cats of an endogenous type C virus with a novel envelope glycoprotein. J. Virol. 53: 827-833, 1985. PubMed: 2983093

    Bassin RH, et al. Normal DBA/2 mouse cells synthesize a glycoprotein which interferes with MCF virus infection. Virology 123: 139-151, 1982. PubMed: 6959413