Mpf (ATCC® CRL-1656)

Organism: Mustela putoris furo, ferret  / 

Organism Mustela putoris furo, ferret
Product Format frozen
Culture Properties adherent
Biosafety Level 1
Age 6 weeks
Applications
transfection host
Comments
Mpf cells have an absolute cloning efficiency greater than 45%.
Complete Growth Medium Basal medium (Eagle) in Earle's BSS, 86%; lamb serum, 14%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended
Medium Renewal: Twice per week
Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes. Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Population Doubling Time 12 to 13 hrs
Name of Depositor RS Trowbridge
References

Trowbridge RS, et al. Establishment and characterization of ferret cells in culture. In Vitro 18: 952-960, 1982. PubMed: 6185411

Basic Documentation
References

Trowbridge RS, et al. Establishment and characterization of ferret cells in culture. In Vitro 18: 952-960, 1982. PubMed: 6185411