2E8 (ATCC® TIB-239)

Organism: Mus musculus, mouse  /  Cell Type: B lymphocyte  /  Tissue: bone marrow  / 

Permits and Restrictions

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Organism Mus musculus, mouse
Tissue bone marrow
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Age immature
Strain BALB/c.xid
Applications
The 2E8 line is dependent on IL-7, and can be used for detecting and quantifying IL-7.
Storage Conditions liquid nitrogen vapor phase
Antigen Expression
Antigen expression: H-2 d; Ia d; BP-1
Receptor Expression
interleukin 7 (IL-7)
Genes Expressed immunoglobulin (surface IgM)
Cellular Products
immunoglobulin (surface IgM)
Comments
The 2E8 line is dependent on IL-7, and can be used for detecting and quantifying IL-7.
Complete Growth Medium Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.05 mM 2-mercaptoethanol, 1 ng/ml mouse interleukin-7 and 20% fetal bovine serum
Subculturing

Every 2 to 3 days, the cells must be diluted without centrifugation at a concentration of 5 X 105 viable cells/mL by adding fresh medium to the culture. 

Mouse IL-7 can be obtained from several commercial sources including Upstate Biotechnology, Inc., Lake Placid, NY (1-800-548-7853). The cells do not grow as well when human IL-7 is substituted for mouse IL-7.

Medium Renewal: Every 2 to 4 days
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Isotype IgM , IgM
Name of Depositor PW Kincade
References

Pietrangeli CE, et al. Stromal cell lines which support lymphocyte growth: characterization, sensitivity to radiation and responsiveness to growth factors. Eur. J. Immunol. 18: 863-872, 1988. PubMed: 3260182

Ishihara K, et al. Stromal-cell and cytokine-dependent lymphocyte clones which span the pre-B- to B-cell transition. Dev. Immunol. 1: 149-161, 1991. PubMed: 1821694

Basic Documentation
Restrictions

1. This hybridoma is provided for research purposes only. Neither the cell line nor the antibody derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purpose of sale, or producing for sale, cells or their products. The cells are provided as a service to the research community. They are provided without warranty or merchantability of fitness for a particular purpose or any other warranty, express or implied. 2. Any proposed commercial use of these cells or antibody produced from them must first be negotiated with the Oklahoma Medical Research Foundation, 825 N.E. 13th, Oklahoma City, OK 73104. Telephone (405) 271-7905.

References

Pietrangeli CE, et al. Stromal cell lines which support lymphocyte growth: characterization, sensitivity to radiation and responsiveness to growth factors. Eur. J. Immunol. 18: 863-872, 1988. PubMed: 3260182

Ishihara K, et al. Stromal-cell and cytokine-dependent lymphocyte clones which span the pre-B- to B-cell transition. Dev. Immunol. 1: 149-161, 1991. PubMed: 1821694