NCI-H146 [H146] (ATCC® HTB-173)

Organism: Homo sapiens, human  /  Cell Type: Epithelial  /  Tissue: lung; derived from metastatic site: bone marrow  /  Disease: carcinoma; small cell lung cancer

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Organism Homo sapiens, human
Tissue lung; derived from metastatic site: bone marrow
Cell Type Epithelial
Product Format frozen
Morphology epithelial
Culture Properties suspension, multicell aggregates
Biosafety Level 1
Disease carcinoma; small cell lung cancer
Age 59 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype This is a near triploid human cell line. The modal chromosome number is 68, but cells with 66, 70 and 71 chromosomes also occurred frequently. At least 15 markers were common to all cells including single copies of der(1)t(1;13)(p32;q12) and del(3)(p21/22), and paired der(7)t(7;?)(p22;?), t(13q16q) and t(16p21q). Ten others were seen in single copies or paired. No HSR or DM were found. Structurally normal N5, N13 and N14 were absent. Four or more copies of N18 and N20 were present in each cell, and N5, N7 and N11 were mostly single copies per cell. The X chromosomes were paired, and no Y chromosome was detected in QM stained preparations.
Clinical Data
59 years
Caucasian
male
Receptor Expression
insulin-like growth factor II (IGF II)
Oncogene myc +; myb +; raf +; ras +; fms +; fes -
Genes Expressed
The cells do not express vasopressin, oxytocin or gastrin releasing peptide.

The cells stain positively for vimentin and keratin, but are negative for neurofilament triplet protein.

There is expression of c-myb, v-fms, Ha-ras, Ki-ras, N-ras and c-raf 1 mRNAs.

Oncogenes: myc +; myb +; raf +; ras +; fms +; fes -

Tumorigenic Yes
Effects
Yes, in nude mice
Comments
The cells produce relatively high amounts of c-myc mRNA, but c-myc DNA sequences are not amplified.
There is expression of c-myb, v-fms, Ha-ras, Ki-ras, N-ras and c-raf 1 mRNAs.
The line expresses elevated levels of four biochemical markers (neuron specific enolase, brain isoenzyme of creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
The line should be subcultured by dilution with fresh medium. Alternatively, the clusters may be collected by centrifugation and resuspended in fresh medium.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11
D13S317: 11,12
D16S539: 11
D5S818: 12
D7S820: 9,10
THO1: 6,9.3
TPOX: 8,11
vWA: 14,16
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
Me-2, 2
PGM1, 1-2
PGM3, 1-2
Name of Depositor AF Gazdar, JD Minna
Year of Origin 1979
References

Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876

Bepler G, et al. Expression of p64c-myc and neuroendocrine properties define three subclasses of small cell lung cancer. Oncogene 4: 45-50, 1989. PubMed: 2536917

Schardt C, et al. Characterization of insulin-like growth factor II receptors in human small cell lung cancer cell lines. Exp. Cell Res. 204: 22-29, 1993. PubMed: 8380141

Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257

Kiefer PE, et al. Amplification and expression of protooncogenes in human small cell lung cancer cell lines. Cancer Res. 47: 6236-6242, 1987. PubMed: 2824028

Hensel CH, et al. Altered structure and expression of the human retinoblastoma susceptibility gene in small cell lung cancer. Cancer Res. 50: 3067-3072, 1990. PubMed: 2159370

Verbeeck MA, et al. Expression of the vasopressin and gastrin-releasing peptide genes in small cell lung carcinoma cell lines. Pathobiology 60: 136-142, 1992. PubMed: 1320893

Lung Cancer 4: 155-161, 1988.

The NCI-H146 cell line was derived by A.F. Gazdar and associates in 1979 from a bone marrow metastasis of a patient with small cell cancer of the lung. The bone marrow specimen was taken prior to therapy.

The cells form transplantable tumors which histologically resemble tumor cells from the original biopsy specimen.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876

Bepler G, et al. Expression of p64c-myc and neuroendocrine properties define three subclasses of small cell lung cancer. Oncogene 4: 45-50, 1989. PubMed: 2536917

Schardt C, et al. Characterization of insulin-like growth factor II receptors in human small cell lung cancer cell lines. Exp. Cell Res. 204: 22-29, 1993. PubMed: 8380141

Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257

Kiefer PE, et al. Amplification and expression of protooncogenes in human small cell lung cancer cell lines. Cancer Res. 47: 6236-6242, 1987. PubMed: 2824028

Hensel CH, et al. Altered structure and expression of the human retinoblastoma susceptibility gene in small cell lung cancer. Cancer Res. 50: 3067-3072, 1990. PubMed: 2159370

Verbeeck MA, et al. Expression of the vasopressin and gastrin-releasing peptide genes in small cell lung carcinoma cell lines. Pathobiology 60: 136-142, 1992. PubMed: 1320893

Lung Cancer 4: 155-161, 1988.

The NCI-H146 cell line was derived by A.F. Gazdar and associates in 1979 from a bone marrow metastasis of a patient with small cell cancer of the lung. The bone marrow specimen was taken prior to therapy.

The cells form transplantable tumors which histologically resemble tumor cells from the original biopsy specimen.