NCI-H929 [H929] (ATCC® CRL-9068)

Organism: Homo sapiens, human  /  Cell Type: B lymphocyte  /  Tissue: bone marrow  /  Disease: plasmacytoma; myeloma

Organism Homo sapiens, human
Tissue bone marrow
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease plasmacytoma; myeloma
Age 62 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype Near tetraploid. Most copies of chromosome 8 have the 8q+ abnormality.
Images
Derivation
This cell line was established from a malignant effusion in a patient with myeloma.
Clinical Data
62 years
Caucasian
female
This cell line was established from a malignant effusion in a patient with myeloma.
Antigen Expression
PCA-1; CD38 +
Receptor Expression
transferrin
Genes Expressed
PCA-1; CD38 +
Comments
The cells are positive for plasma cell antigen 1 (PCA-1), transferrin receptor, CD38 (T10), but are negative for HLA DR, CALLA and markers of early B cell development.
They are negative for Epstein-Barr virus nuclear antigen (EBNA).
The cells have a rearrangement of the c-myc proto oncogene and express c-myc RNA.
There is also an activated ras allele.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 4 X 105 viable cells/mL. Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product.
Interval: Maintain cultures at cell concentrations between 5 X 105 and 1 X 106 viable cells/mL.
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11
D13S317: 12
D16S539: 9,13
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 8,11
vWA: 14,15
Isotype IgA kappa (Recent tests for IgA, kappa have not detected the production of IgA; the cells are producing kappa light chain.)
Name of Depositor National Cancer Institute
References

Hollis GF, et al. Complex translocation disrupts c-myc regulation in a human plasma cell myeloma. Mol. Cell. Biol. 8: 124-129, 1988. PubMed: 3275865

Gazdar AF, et al. Establishment and characterization of a human plasma cell myeloma culture having a rearranged cellular myc proto-oncogene. Blood 67: 1542-1549, 1986. PubMed: 2423157

Ernst TJ, et al. Identification of a second transforming gene, rasn, in a human multiple myeloma line with a rearranged c-myc allele. Blood 72: 1163-1167, 1988. PubMed: 3048435

Recent tests for IgA, kappa have not detected the production of IgA; the cells are producing kappa light chain.

Recent tests for IgA, kappa have not detected the production of IgA; the cells are producing kappa light chain.

Basic Documentation
Other Documentation
References

Hollis GF, et al. Complex translocation disrupts c-myc regulation in a human plasma cell myeloma. Mol. Cell. Biol. 8: 124-129, 1988. PubMed: 3275865

Gazdar AF, et al. Establishment and characterization of a human plasma cell myeloma culture having a rearranged cellular myc proto-oncogene. Blood 67: 1542-1549, 1986. PubMed: 2423157

Ernst TJ, et al. Identification of a second transforming gene, rasn, in a human multiple myeloma line with a rearranged c-myc allele. Blood 72: 1163-1167, 1988. PubMed: 3048435

Recent tests for IgA, kappa have not detected the production of IgA; the cells are producing kappa light chain.

Recent tests for IgA, kappa have not detected the production of IgA; the cells are producing kappa light chain.