NCI-H711 [H711] (ATCC® CRL-5836_FL)

Organism: Homo sapiens, human  /  Tissue: Carcinoma; classic small cell lung cancer; lung  /  Disease: stage E, carcinoma; classic small cell lung cancer

Permits and Restrictions

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Organism Homo sapiens, human
Tissue Carcinoma; classic small cell lung cancer; lung
Product Format flask
Morphology rounded
Culture Properties suspension, aggregates of round cell clusters
Biosafety Level 1
Disease stage E, carcinoma; classic small cell lung cancer
Age 49 years
Gender male
Ethnicity Caucasian
Derivation
The line was established in February 1984.
Derived from metastatic site: bone marrow
Clinical Data
male
Caucasian
49 years
Comments
The patient was a smoker.
60 pack years.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Procedure: Cultures can be maintained by the addition of fresh medium.  Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 106 viable cells/mL.  Maintain cell density between 106 and 3 x 106 cells/mL.

Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density).

Cryopreservation
Culture medium, 95%; DMSO, 5%
Name of Depositor AF Gazdar, JD Minna
Year of Origin 1984
References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Basic Documentation
Restrictions

The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.

References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.