VA-ES-BJ (ATCC® CRL-2138)

Organism: Homo sapiens, human  /  Tissue: derived from metastatic site: bone marrow  /  Disease: epithelioid sarcoma

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Organism Homo sapiens, human
Tissue derived from metastatic site: bone marrow
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease epithelioid sarcoma
Age 41 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype near triploid karyotype with structural as well as numerical abnormalities; modal number = 65. Rearrangements of both chromosomes 1 and 9 with unknown materials were present., Five copies of chromosome 7 were seen in almost every cell, and there was no intact Y chromosome present. Cell to cell variation was due to numerical differences only, structural abnormalities were very consistent.
Derivation
VA-ES-BJ is an epithelioid sarcoma cell line derived in 1991 from a bone marrow metastasis removed from an adult male with primary vertebral epithelioid carcinoma.
Clinical Data 41 years
male
Caucasian
Oncogene p53 +
Genes Expressed
Granulocyte macrophage colony stimulating factor (GM-CSF)
Tumorigenic Yes
Effects
Yes, forms tumors in nude mice
Comments

The cells are sensitive to hemin.

The cell are positive for immunohistochemical staining for the following: AE-1 and AE-3 cytokeratins, fas, epithelial membrane antigen (EMA), vimentin and p53.

Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose and 0.1 mM non-essential amino acids; fetal bovine serum, 10%
Subculturing Volumes used in this protocol are for 75cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25%Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels
  6. Place culture vessels in incubators at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended
Medium Renewal: Twice per week

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells: a Manual of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005.

Cryopreservation
Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC® Catalog No. 4-X.
Name of Depositor C Helson
Year of Origin 1991
References

Helson C, et al., Va-es-bj - an epithelioid sarcoma cell-line. Int. J. Oncol. 7: 51-56, 1995. PubMed: 21552805

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Helson C, et al., Va-es-bj - an epithelioid sarcoma cell-line. Int. J. Oncol. 7: 51-56, 1995. PubMed: 21552805

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.