SR-4987 (ATCC® CRL-2028)

Organism: Mus musculus, mouse  /  Cell Type: virus transformed  /  Disease: Leukemia

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Organism Mus musculus, mouse
Cell Type virus transformed
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 2
Disease Leukemia
Age 5 months
Gender female
Strain BDF1
Applications
transfection host
Karyotype tetraploid
Derivation
The SR-4987 cell line was established by treating normal marrow cells with supernatant from Y-1 cells (known to produce Murine Leukemia Virus (MuLV).
Clinical Data
female
Genes Expressed
macrophage colony stimulating factor (MCSF)
Tumorigenic Yes
Effects
Yes, produce sarcomas in syngeneic mice
Comments
The SR-4987 cell line was established by treating normal marrow cells with supernatant from Y-1 cells (known to produce Murine Leukemia Virus (MuLV).
The cells produce MCSF, and are highly sensitive to fibroblast growth factor (FGF).
They are poorly clonogenic in soft agar.
Neither interleukin 3 (IL-3) or granulocyte colony stimulating factor (GCSF) were detected in SR-4987 conditioned medium.
Complete Growth Medium The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove the trypsin.
Add fresh trypsin solution (1 to 2 ml), and allow the culture to sit at room temperature (or at 37C) until the cells begin to detach.
Add fresh medium, aspirate and dispense into new flasks.
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Population Doubling Time 15 hrs
Name of Depositor G Zaleskis
References

Pessina A, et al. Establishment and characterization of a new murine cell line (SR-4987) derived from marrow stromal cells. Cytotechnology 8: 93-102, 1992. PubMed: 1382506

Pessina A, et al. Expression of B cell markers on SR-4987 cells derived from murine bone marrow stroma. Exp. Hematol. 25: 536-541, 1997. PubMed: 9197333

Pessina A, et al. Role of SR-4987 stromal cells in the modulation of doxorubicin toxicity to in vitro granulocyte-macrophage progenitors (CFU-GM). Life Sci. 65: 513-523, 1999. PubMed: 10462078

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Pessina A, et al. Establishment and characterization of a new murine cell line (SR-4987) derived from marrow stromal cells. Cytotechnology 8: 93-102, 1992. PubMed: 1382506

Pessina A, et al. Expression of B cell markers on SR-4987 cells derived from murine bone marrow stroma. Exp. Hematol. 25: 536-541, 1997. PubMed: 9197333

Pessina A, et al. Role of SR-4987 stromal cells in the modulation of doxorubicin toxicity to in vitro granulocyte-macrophage progenitors (CFU-GM). Life Sci. 65: 513-523, 1999. PubMed: 10462078