D1 ORL UVA [D1] (ATCC® CRL-12424)

Organism: Mus musculus, mouse  /  Tissue:

bone marrow


 / 

Organism Mus musculus, mouse
Tissue

bone marrow


Product Format frozen
Culture Properties adherent
Biosafety Level 1
Strain BALB/c
Applications This cell line is a suitable transfection host. 

The cells may be systemically administered to treat osteoporosis, osteolysis, improve bone implant adherence, augment bone growth or bone repair, augment cartilage repair, and augment fat production.

Storage Conditions liquid nitrogen vapor phase
Derivation
The D1 cell line was cloned from a multipotent mouse bone marrow stromal precursor. 
Clinical Data
The cells are "homing" cells, that is, cells injected systemically into the patient home to the bone marrow, unless specifically placed in another location.
Comments

The cells can differentiate into osteocytes, chondrocytes and adipocytes in the presense of the appropriate stimulus and environment.

The cells are "homing" cells, that is, cells injected systemically into the patient home to the bone marrow, unless specifically placed in another location.

The cells may be transformed with recombinant DNA for the expression of both reporter genes, and biological factors, such as growth factors.


Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
    Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
    Medium Renewal: Every 2 to 3 days
    Cryopreservation
    Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
    Storage temperature: liquid nitrogen vapor phase
    Culture Conditions
    Temperature: 37°C
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Name of Depositor University of Virginia
    References

    Balian G, et al. Pluripotential bone marrow cell line and methods of using the same. US Patent 6,082,364 dated Jul 4 2000

    Basic Documentation
    References

    Balian G, et al. Pluripotential bone marrow cell line and methods of using the same. US Patent 6,082,364 dated Jul 4 2000