EML Cell Line, Clone 1 (ATCC® CRL-11691)

Organism: Mus musculus, mouse  /  Cell Type: basophil  /  Tissue: bone marrow  / 

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Organism Mus musculus, mouse
Tissue bone marrow
Cell Type basophil
Product Format frozen
Culture Properties suspension
Biosafety Level 2 [Cells contain SV-40 viral DNA sequences]
Gender male
Strain BDF1
Storage Conditions liquid nitrogen vapor phase
Derivation

Bone marrow cells of male BDF1 mice were infected with the retroviral vector LRARalpha403SN. RARalpha403 was inserted into the retroviral vector LXSN to generate the recombinant vector designated LRARalpha403SN. 

The infected cells were initially grown in media containing WEHI 3B conditioned medium as a source of murine IL-3), murine GM-CSF, human IL-1beta, human IL-6 and polybrene.

Clinical Data
male
Comments

EML Clone 1 is a stem cell factor-dependent lympho-hematopoietic progenitor cell line. They can be induced with IL-7 and bone marrow stromal cells to express characteristics of pre-pro-B lymphocytes.

EML contains progenitors capable of differentiation along B-lymphocyte, erythrocyte, neutrophil, macrophage, mast cell, and megakaryocyte lineages. They are the only known SCF-dependent cell line with both lympho and myelo-erythroid potential.

These cells are available only as a frozen ampule.

RARalpha403 is a truncated cDNA that encodes a peptide of 403 amino acids containing the N-terminus and the DNA-binding domain. RARalphacDNA contains a truncation of the sequences coding for the C-terminal 59 amino acids as well as a portion of the 3' untranslated region. 


Complete Growth Medium Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate containing 200 ng/ml mouse stem cell factor, 80%; heat-inactivated fetal bovine serum, 20%
Subculturing
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 2 to 3 x 105 viable cells/mL.
Interval: Maintain cultures at cell concentrations between 4 x105 and 2 x 106 viable cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Name of Depositor Fred Hutchinson Cancer Res. Cntr.
References

Weiler SR, et al. D3: a gene induced during myeloid cell differentiation of Linlo c-Kit+ Sca-1(+) progenitor cells. Blood 93: 527-536, 1999. PubMed: 9885214

Tsai S, Collins SJ. Creating novel hematopoietic cell lines by expressing altered retinoic acid receptors. US Patent 5,830,760 dated Nov 3 1998

Johnson BS, et al. Retinoid X receptor (RXR) agonist-induced activation of dominant-negative RXR-retinoic acid receptor alpha403 heterodimers is developmentally regulated during myeloid differentiation. Mol. Cell. Biol. 19: 3372-3382, 1999. PubMed: 10207061

Du Y, et al. Identification of an interleukin-3-regulated aldoketo reductase gene in myeloid cells which may function in autocrine regulation of myelopoiesis. J. Biol. Chem. 275: 6724-6732, 2000. PubMed: 10702227

Lawson ND, et al. Normal neutrophil differentiation and secondary granule gene expression in the EML and MPRO cell lines. Exp. Hematol. 26: 1178-1185, 1998. PubMed: 9808058

Lawson ND, et al. Isolation and characterization of the cDNA for mouse neutrophil collagenase: demonstration of shared negative regulatory pathways for neutrophil secondary granule protein gene expression. Blood 91: 2517-2524, 1998. PubMed: 9516153

Lawson ND, Berliner N. Representational difference analysis of a committed myeloid progenitor cell line reveals evidence for bilineage potential. Proc. Natl. Acad. Sci. USA 95: 10129-10133, 1998. PubMed: 9707612

Aoyama K, et al. Stromal cell CD9 regulates differentiation of hematopoietic stem/progenitor cells. Blood 15: 2586-2594, 1999. PubMed: 10194438

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Weiler SR, et al. D3: a gene induced during myeloid cell differentiation of Linlo c-Kit+ Sca-1(+) progenitor cells. Blood 93: 527-536, 1999. PubMed: 9885214

Tsai S, Collins SJ. Creating novel hematopoietic cell lines by expressing altered retinoic acid receptors. US Patent 5,830,760 dated Nov 3 1998

Johnson BS, et al. Retinoid X receptor (RXR) agonist-induced activation of dominant-negative RXR-retinoic acid receptor alpha403 heterodimers is developmentally regulated during myeloid differentiation. Mol. Cell. Biol. 19: 3372-3382, 1999. PubMed: 10207061

Du Y, et al. Identification of an interleukin-3-regulated aldoketo reductase gene in myeloid cells which may function in autocrine regulation of myelopoiesis. J. Biol. Chem. 275: 6724-6732, 2000. PubMed: 10702227

Lawson ND, et al. Normal neutrophil differentiation and secondary granule gene expression in the EML and MPRO cell lines. Exp. Hematol. 26: 1178-1185, 1998. PubMed: 9808058

Lawson ND, et al. Isolation and characterization of the cDNA for mouse neutrophil collagenase: demonstration of shared negative regulatory pathways for neutrophil secondary granule protein gene expression. Blood 91: 2517-2524, 1998. PubMed: 9516153

Lawson ND, Berliner N. Representational difference analysis of a committed myeloid progenitor cell line reveals evidence for bilineage potential. Proc. Natl. Acad. Sci. USA 95: 10129-10133, 1998. PubMed: 9707612

Aoyama K, et al. Stromal cell CD9 regulates differentiation of hematopoietic stem/progenitor cells. Blood 15: 2586-2594, 1999. PubMed: 10194438