SW 1353 [SW 1353, SW-1353] (ATCC® HTB-94)

Organism: Homo sapiens, human  /  Tissue: bone  /  Disease: chondrosarcoma

Organism Homo sapiens, human
Tissue
bone
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease chondrosarcoma
Age 72 years
Gender female
Ethnicity Caucasian
Applications
transfection host
Storage Conditions liquid nitrogen vapor temperature
Karyotype hyperdiploid 47, XX, +7; Except for the trisomic N7 no other chromosome markers are evident
Derivation
The SW 1353 cell line was initiated by A. Leibovitz at the Scott and White Clinic, Temple, Texas in 1977 from a primary grade II chondrosarcoma of the right humerus obtained from a 72 year old female Caucasian.
Clinical Data
72 years
Caucasian
female
The SW 1353 cell line was initiated by A. Leibovitz at the Scott and White Clinic, Temple, Texas in 1977 from a primary grade II chondrosarcoma of the right humerus obtained from a 72 year old female Caucasian.
Antigen Expression
Antigen expression: Blood type O-
Comments
The initial culture medium was L-15 containing cortisone and insulin plus 10% fetal bovine serum and antibiotics.
A frozen ampule at passage 12 was received at the ATCC in January, 1982.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37°C
Atmosphere: air, 100%
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 12,13
D16S539: 11,12
D5S818: 10,11
D7S820: 9,11
THO1: 6,9
TPOX: 8,11
vWA: 16,17
Isoenzymes
AK-1, 1
ES-D, 2
G6PD, B
GLO-I, 2
PGM1, 1
PGM3, 2
Name of Depositor A Leibovitz
Passage History
A frozen ampule at passage 12 was received at the ATCC in January, 1982.