SK-ES-1 (ATCC® HTB-86)

Organism: Homo sapiens, human  /  Tissue: bone  /  Disease: sarcoma (anaplastic osteosarcoma or Ewing's sarcoma)

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Organism Homo sapiens, human
Tissue
bone
Product Format frozen
Morphology epithelial
Biosafety Level 1
Disease sarcoma (anaplastic osteosarcoma or Ewing's sarcoma)
Age 18 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 49; range = 44 to 51. This is a hyperdiploid human cell line with the modal chromosome number of 49 occurring in 34% of cells. However, cells with 50 chromosomes also occurred at high percentage (24%). At least 11 markers were consistently present in most cells. Among them were: der(3)t(3;12) (q21;q13); del(3) (q21); inv(1) (p36.1q21), and der(16)t(5;16) (q13;p13.3), all present in single copy per cell. At least three Y-autosome markers were detected. Single normal X chromosome was found in every cell. Normal Y chromosome was not found.
Clinical Data
18 years
Antibodies to the sarcoma lines were detected in sera of patients with sarcomas after absorption with non-sarcoma lines.
Caucasian
male
Sera from normal donors were also cytotoxic.
Antigen Expression
Antigen expression: Blood Type A; Rh+
Tumorigenic Yes
Effects
Yes, in nude mice, forms small cell malignant tumor consistent with Ewing sarcoma
Comments
Antibodies to the sarcoma lines were detected in sera of patients with sarcomas after absorption with non-sarcoma lines. Sera from normal donors were also cytotoxic.
Complete Growth Medium The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified , Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
STR Profile
Amelogenin: X,Y
CSF1PO: 11
D13S317: 8,9
D16S539: 11
D5S818: 12
D7S820: 10,11
THO1: 6,9.3
TPOX: 8
vWA: 14,17
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
PGM1, 1
PGM3, 1-2
Name of Depositor G Trempe, LJ Old
Year of Origin 1971
References

Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975.

Bloom ET. Further definition by cytotoxicity tests of cell surface antigens of human sarcomas in culture. Cancer Res. 32: 960-967, 1972. PubMed: 4502173

Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

Basic Documentation
Restrictions

The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the cells subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with the Office of Technology Development, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065. Contact Tingting Zhang-Kharas, Direct Phone: 646-888-1083, Reception: 646-888-1080, Email: zhangkht@mskcc.org

References

Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975.

Bloom ET. Further definition by cytotoxicity tests of cell surface antigens of human sarcomas in culture. Cancer Res. 32: 960-967, 1972. PubMed: 4502173

Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871