DSN (ATCC® CRL-9939)

Organism: Canis familiaris  /  Disease: osteosarcoma

Organism Canis familiaris
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 Cells contain CMV, SNV, SV-40 viral DNA sequences
Disease osteosarcoma
Age 11 years
Gender female
Strain poodle
Applications
The DSN cell line was derived from D-17 (see ATCC CCL-183) cells by transfecting with plasmids containing the gag-pol and the env genes of spleen necrosis virus.
The line is for use as a helper cell for propagating replication incompetent vectors derived from the SNV retrovirus.
Derivation
The DSN cell line was derived from D-17 (see ATCC CCL-183) cells by transfecting with plasmids containing the gag-pol and the env genes of spleen necrosis virus.
The line is for use as a helper cell for propagating replication incompetent vectors derived from the SNV retrovirus.
Clinical Data
female
Comments
The DSN cell line was derived from D-17 (see ATCC CCL-183) cells by transfecting with plasmids containing the gag-pol and the env genes of spleen necrosis virus.
The line is for use as a helper cell for propagating replication incompetent vectors derived from the SNV retrovirus.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:12 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, add fresh 0.25% trypsin-0.02% EDTA for several minutes, remove trypsin and let the culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 4 to 6 days.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor Wisconsin Alumni Res. Fndn.
References

Temin HM, Dougherty JP. Recombination resistant retroviral helper cell and products produced thereby. US Patent 5,124,263 dated Jun 23 1992

Basic Documentation
References

Temin HM, Dougherty JP. Recombination resistant retroviral helper cell and products produced thereby. US Patent 5,124,263 dated Jun 23 1992