143B (ATCC® CRL-8303)

Organism: Homo sapiens, human  /  Tissue: bone  /  Disease: osteosarcoma

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Organism Homo sapiens, human
Tissue bone
Product Format frozen
Morphology mixed
Culture Properties adherent
Biosafety Level 1
Disease osteosarcoma
Age 13 year old
Gender female
Ethnicity Caucasian
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Clinical Data
13 year old
Caucasian
female
Comments
Thymidine kinase negative (TK-).
This is a human osteosarcoma cell line.
Complete Growth Medium Minimum essential medium (Eagle) in Earle's BSS with 0.015 mg/ml 5-bromo-2'-deoxyuridine, 90%; fetal bovine serum, 10%
Subculturing
Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution and allow the cells to sit at room temperature (or at 37°C) until they detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Medium Renewal: 2 to 3 times per week
Cryopreservation
90% FBS; 10% DMSO
Culture Conditions
Temperature: 37°C

STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 11
vWA: 18
Name of Depositor Wistar Institute
References

Berson JF, et al. A seven-transmembrane domain receptor involved in fusion and entry of T-cell-tropic human immunodeficiency virus tyep 1 strains. J. Virol. 70: 6288-6295, 1996. PubMed: 8709256

Roller RJ, et al. Structure and function in the herpes simplex virus 1 RNA-binding protein US11: mapping of the domain required for ribosomal and nucleolar association and RNA binding in vitro. J. Virol. 70: 2842-2851, 1996. PubMed: 8627758

Hofhaus G, et al. Respiration and growth defects in transmitochondrial cell lines carrying the 11778 mutation associated with Leber's hereditary optic neuropathy. J. Biol. Chem. 271: 13155-13161, 1996. PubMed: 8662757

Hocking AM, et al. Eukaryotic expression of recombinant biglycan. J. Biol. Chem. 271: 19571-19577, 1996. PubMed: 8702651

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
FAQ's
  1. Adding 5-bromo-2’-deoxyuridine to CRL-8303 medium


    Date Updated: 8/19/2013

References

Berson JF, et al. A seven-transmembrane domain receptor involved in fusion and entry of T-cell-tropic human immunodeficiency virus tyep 1 strains. J. Virol. 70: 6288-6295, 1996. PubMed: 8709256

Roller RJ, et al. Structure and function in the herpes simplex virus 1 RNA-binding protein US11: mapping of the domain required for ribosomal and nucleolar association and RNA binding in vitro. J. Virol. 70: 2842-2851, 1996. PubMed: 8627758

Hofhaus G, et al. Respiration and growth defects in transmitochondrial cell lines carrying the 11778 mutation associated with Leber's hereditary optic neuropathy. J. Biol. Chem. 271: 13155-13161, 1996. PubMed: 8662757

Hocking AM, et al. Eukaryotic expression of recombinant biglycan. J. Biol. Chem. 271: 19571-19577, 1996. PubMed: 8702651