SJCRH30 [RC13, RMS 13, SJRH30] (ATCC® CRL-2061)

Organism: Homo sapiens, human  /  Tissue: muscle; derived from metastatic site: bone marrow  /  Disease: rhabdomyosarcoma

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Organism Homo sapiens, human
Tissue muscle; derived from metastatic site: bone marrow
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease rhabdomyosarcoma
Age 17 years
Gender male
Ethnicity Caucasian
Derivation
The line was established from cells from the bone marrow of a child with rhabdomyosarcoma.
Derived from metastatic site, bone marrow
Clinical Data
17 years
Caucasian
male

Oncogene gli + (amplified 30 fold), N-myc + (not amplified)
Genes Expressed
gli + (amplified 30 fold), N-myc + (not amplified)
Comments
The cells show ultrastructural elements of primitive skeletal muscle differentiation.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended
Medium Renewal: Twice per week
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
D5S818: 12, 13
D13S317: 11
D7S820: 10
D16S539: 12
THO1: 9, 9.3
TPOX: 8, 11
vWA: 17, 18
CSF1PO: 10, 11
Amelogenin: XY
Name of Depositor DN Shapiro
References

Oliner JD, et al. Amplification of a gene encoding a p53-associated protein in human sarcomas. Nature 358: 80-83, 1992. PubMed: 1614537

Roberts WM, et al. Amplification of the gli gene in childhood sarcomas. Cancer Res. 49: 5407-5413, 1989. PubMed: 2766305

Douglass EC, et al. A specific chromosomal abnormality in rhabdomyosarcoma [published erratum appears in Cytogenet Cell Genet 1988;47(4):following 232]. Cytogenet. Cell Genet. 45: 148-155, 1987. PubMed: 3691179

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Oliner JD, et al. Amplification of a gene encoding a p53-associated protein in human sarcomas. Nature 358: 80-83, 1992. PubMed: 1614537

Roberts WM, et al. Amplification of the gli gene in childhood sarcomas. Cancer Res. 49: 5407-5413, 1989. PubMed: 2766305

Douglass EC, et al. A specific chromosomal abnormality in rhabdomyosarcoma [published erratum appears in Cytogenet Cell Genet 1988;47(4):following 232]. Cytogenet. Cell Genet. 45: 148-155, 1987. PubMed: 3691179