C13589 (ATCC® CRL-2704)

Organism: Homo sapiens, human  /  Cell Type: B lymphoblast; immortalized with Epstein-Barr virus (EBV)Epstein-Barr virus (EBV) transforme  / 

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Organism Homo sapiens, human
Cell Type B lymphoblast; immortalized with Epstein-Barr virus (EBV)Epstein-Barr virus (EBV) transforme
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 Cells contain EBV viral DNA sequences

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age 39 years adult
Gender female
Ethnicity Caucasian
Applications
This cell line can be used a standard for sizing.
Storage Conditions -135°C
Clinical Data
female
Caucasian
39 years adult
Comments
This cell line can be used a standard for sizing.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 10 exp5 viable cells/ml. Maintain cell density between 3 X 10 exp5 and 3 X 10 exp6 viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: -135°C
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 8,12
D16S539: 9,10
D5S818: 12,13
D7S820: 9
THO1: 6,8
TPOX: 10,11
vWA: 15
Name of Depositor SL Nolin
References

The line was established from mononuclear cells taken from a fragile-X premutation female with 31 and 59 CGG-triplet repeats in the fragile-X gene, FMR1. This allele size is the smallest undergoing expansion to a full mutation (greater than 200 repeats) in one transmission. This expansion to a full mutation with greater than 200 repeats occurs only on transmission through females. Fragile X is a common cause of inherited mental retardation and is caused by an expansion of a CGG repeat within the gene.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

The line was established from mononuclear cells taken from a fragile-X premutation female with 31 and 59 CGG-triplet repeats in the fragile-X gene, FMR1. This allele size is the smallest undergoing expansion to a full mutation (greater than 200 repeats) in one transmission. This expansion to a full mutation with greater than 200 repeats occurs only on transmission through females. Fragile X is a common cause of inherited mental retardation and is caused by an expansion of a CGG repeat within the gene.