BCP-1 (ATCC® CRL-2294)

Organism: Homo sapiens, human  /  Cell Type: B lymphoblast  /  Disease: body cavity based lymphoma

Organism Homo sapiens, human
Cell Type B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 CELLS CONTAIN HERPESVIRUS
Disease body cavity based lymphoma
Age 92 years
Gender male
Applications
The BCP-1 cell line is positive for the Kaposi's sarcoma associated herpesvirus (KSHV, provisionally designated Human Herpes Virus-8 or HHV-8) genome at 150 copies/cell.
BCP-1 is negative for Epstein-Barr virus (EBV), HHV-6 and HHV-7.
Storage Conditions liquid nitrogen vapor phase
Derivation
BCP-1 is a clonal lymphoma cell line isolated in 1995 by serial dilution of peripheral blood mononuclear cells from an HIV seronegative patient with body cavity based lymphoma (BCBL).
Clinical Data
BCP-1 is a clonal lymphoma cell line isolated in 1995 by serial dilution of peripheral blood mononuclear cells from an HIV seronegative patient with body cavity based lymphoma (BCBL).
male
Comments
BCP-1 is a clonal lymphoma cell line isolated in 1995 by serial dilution of peripheral blood mononuclear cells from an HIV seronegative patient with body cavity based lymphoma (BCBL).
The BCP-1 cell line is positive for the Kaposi's sarcoma associated herpesvirus (KSHV, provisionally designated Human Herpes Virus-8 or HHV-8) genome at 150 copies/cell.
BCP-1 is negative for Epstein-Barr virus (EBV), HHV-6 and HHV-7.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml.
Interval: Maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Name of Depositor SJ Gao, PS Moore, Y Chang
Year of Origin 1995
References

Gao SJ, et al. KSHV antibodies among Americans, Italians and Ugandans with and without Kaposi's sarcoma. Nat. Med. 2: 925-928, 1996. PubMed: 8705864

Basic Documentation
References

Gao SJ, et al. KSHV antibodies among Americans, Italians and Ugandans with and without Kaposi's sarcoma. Nat. Med. 2: 925-928, 1996. PubMed: 8705864