COLO 829BL (ATCC® CRL-1980)

Organism: Homo sapiens, human  /  Cell Type: B lymphoblast; Epstein-Barr virus (EBV) transformed  /  Tissue: peripheral blood  /  Disease: normal

Organism Homo sapiens, human
Tissue peripheral blood
Cell Type B lymphoblast; Epstein-Barr virus (EBV) transformed
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2  [Cells contain Herpesvirus]
Disease normal
Age 45 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Derivation

The specimen was taken from a patient prior to chemotherapy for metastatic melanoma.

The COLO 829BL cell line was established at the ATCC by Epstein-Barr virus (EBV) transformation of of a sample of peripheral blood provided by G.E. Moore.

A cell line was also produced from the melanoma, and is available as COLO 829 (ATCC CRL-1974).
Clinical Data
45 years
male
Caucasian
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 4 x 105 viable cells/mL. Maintain cell density between 2 x 105 and 2 x 106 viable cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation Complete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC® Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Name of Depositor GE Moore
References

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Basic Documentation
References

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.