FOX-NY (ATCC® CRL-1732)

Organism: Mus musculus, mouse  /  Cell Type: B lymphoblast  / 

Organism Mus musculus, mouse
Cell Type B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Strain BALB/c
Applications
FOX-NY was derived from the NS-1 myeloma cell line.
It is resistant to analogs of both adenosine and guanine (e.
When used as the immortal partner in a fusion using B cells from a female mouse with the Rb(8.12) translocation, there is a positive selection for the immunoglobulin heavy chain locus.
Selection for the Igh locus results in more stable antibody production in the resulting hybridomas.
Tested and found negative for ectromelia virus (mousepox).
Derivation
FOX-NY was derived from the NS-1 myeloma cell line.
Clinical Data
When used as the immortal partner in a fusion using B cells from a female mouse with the Rb(8.12) translocation, there is a positive selection for the immunoglobulin heavy chain locus.
Comments
FOX-NY was derived from the NS-1 myeloma cell line.
It is resistant to analogs of both adenosine and guanine (e.g., 2,6-diaminopurine and 8-azaguanine) as a result of deficiencies in hypoxanthine phosphoribosyl transferase (HPRT, HGPRT) and adenosine phosphoribosyl transferase (APRT).
When used as the immortal partner in a fusion using B cells from a female mouse with the Rb(8.12) translocation, there is a positive selection for the immunoglobulin heavy chain locus.
If the fusion products are grown in the presence of aminopterin, adenine and thymidine (or azaserine and adenine).
Selection for the Igh locus results in more stable antibody production in the resulting hybridomas.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor RT Taggart
References

Taggart RT, Samloff IM. Stable antibody-producing murine hybridomas. Science 219: 1228-1230, 1983. PubMed: 6402815

Basic Documentation
References

Taggart RT, Samloff IM. Stable antibody-producing murine hybridomas. Science 219: 1228-1230, 1983. PubMed: 6402815