RPMI 7666 [Gerner 7666] (ATCC® CCL-114)

Organism: Homo sapiens, human  /  Cell Type: B lymphoblast  / 

Permits and Restrictions

View Permits

Organism Homo sapiens, human
Cell Type B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 Cells Contain HERPESVIRUS

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age 32 years
Gender male
Ethnicity Caucasian
Applications
The cells produce Epstein-Barr virus.
Clinical Data
male
Caucasian
32 years
Antigen Expression
HLA A3, Aw29, B7, B12; Blood type B+
Genes Expressed
immunoglobulin; Epstein-Barr virus (EBV),HLA A3, Aw29, B7, B12; Blood type B+
Cellular Products
immunoglobulin; Epstein-Barr virus (EBV)
Comments
Biological Safety CLASS II.
EBNA positive.
The cells produce Epstein-Barr virus.
Complete Growth Medium RPMI 1640 medium, 80%; fetal bovine serum, 20%
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by addition or replacement of medium. Establish new cultures at 5 X 10 exp5 viable cells/ml.
STR Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,12
D16S539: 9,11
D5S818: 11,12
D7S820: 8,11
THO1: 7,9.3
TPOX: 8,11
vWA: 14,16
Name of Depositor GE Moore
References

Moore GE, et al. Culture of normal human leukocytes. JAMA 199: 519-524, 1967. PubMed: 4960081

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Moore GE, et al. Culture of normal human leukocytes. JAMA 199: 519-524, 1967. PubMed: 4960081