RPMI 7666 [Gerner 7666] (ATCC® CCL-114)

Organism: Homo sapiens, human  /  Cell Type: B lymphoblast  / 

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Organism Homo sapiens, human
Cell Type B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 Cells Contain HERPESVIRUS
Age 32 years
Gender male
Ethnicity Caucasian
Applications
The cells produce Epstein-Barr virus.
Clinical Data
male
Caucasian
32 years
Antigen Expression
HLA A3, Aw29, B7, B12; Blood type B+
Genes Expressed
immunoglobulin; Epstein-Barr virus (EBV),HLA A3, Aw29, B7, B12; Blood type B+
Comments
Biological Safety CLASS II.
EBNA positive.
The cells produce Epstein-Barr virus.
Complete Growth Medium RPMI 1640 medium, 80%; fetal bovine serum, 20%
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by addition or replacement of medium. Establish new cultures at 5 X 10 exp5 viable cells/ml.
STR Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,12
D16S539: 9,11
D5S818: 11,12
D7S820: 8,11
THO1: 7,9.3
TPOX: 8,11
vWA: 14,16
Name of Depositor GE Moore
References

Moore GE, et al. Culture of normal human leukocytes. JAMA 199: 519-524, 1967. PubMed: 4960081

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Moore GE, et al. Culture of normal human leukocytes. JAMA 199: 519-524, 1967. PubMed: 4960081