CPA 47 (ATCC® CRL-1733)

Organism: Bos taurus, cow  /  Cell Type: endothelial  / 

Organism Bos taurus, cow
Cell Type endothelial
Product Format frozen
Morphology cobblestone
Culture Properties adherent
Biosafety Level 1
Age calf
Applications
The CPA 47 cell line was established from cells scraped from the luminal surface of the pulmonary artery.
CPA 47 cells show cobblestone morphology, and are positive for factor VIII and angiotensin converting enzyme (ACE);
Recent tests for bovine viral diarrhea (BVD) virus indicate that CPA 47 cells are positive for both BVD antigen and infectious virions when the culture medium contains serum of bovine origin.
Derivation
The CPA 47 cell line was established from cells scraped from the luminal surface of the pulmonary artery.
Genes Expressed
Tested and found negative for ectromelia virus (mousepox).
factor VIII; angiotensin converting enzyme,Recent tests for bovine viral diarrhea (BVD) virus indicate that CPA 47 cells are positive for both BVD antigen and infectious virions when the culture medium contains serum of bovine origin.
Cellular Products
factor VIII; angiotensin converting enzyme
Comments
The CPA 47 cell line was established from cells scraped from the luminal surface of the pulmonary artery.
The cells have never been exposed to proteolytic enzymes, and therefore possess an intact glycocalyx.
CPA 47 cells show cobblestone morphology, and are positive for factor VIII and angiotensin converting enzyme (ACE);
The cells have a life expectancy of about 5 years (120 passages), and can be grown on microcarriers;
Recent tests for bovine viral diarrhea (BVD) virus indicate that CPA 47 cells are positive for both BVD antigen and infectious virions when the culture medium contains serum of bovine origin.
Current lots have been propagated using horse serum and all tests for BVD virus were negative.
Complete Growth Medium Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 90%; horse serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Twice per week
Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells from the substrate, aspirate and dispense into new flasks new flasks.
Cryopreservation
culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor US Ryan
Passage History
The cells have a life expectancy of about 5 years (120 passages), and can be grown on microcarriers;
References

Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438

Ryan US, et al. Isolation and culture of pulmonary artery endothelial cells. Tissue Cell 10: 535-554, 1978. PubMed: 83028

Ryan US, et al. Methods for microcarrier culture of bovine pulmonary artery endothelial cells avoiding the use of enzymes. Tissue Cell 12: 619-635, 1980. PubMed: 7010675

Basic Documentation
References

Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438

Ryan US, et al. Isolation and culture of pulmonary artery endothelial cells. Tissue Cell 10: 535-554, 1978. PubMed: 83028

Ryan US, et al. Methods for microcarrier culture of bovine pulmonary artery endothelial cells avoiding the use of enzymes. Tissue Cell 12: 619-635, 1980. PubMed: 7010675