CPAE (ATCC® CCL-209)

Organism: Bos taurus, cow  /  Cell Type: endothelial  /  Tissue: pulmonary artery/endothelium  / 

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Organism Bos taurus, cow
Tissue pulmonary artery/endothelium
Cell Type endothelial
Product Format frozen
Morphology endothelial
Culture Properties adherent
Biosafety Level 2 [Cells contain Bovine Viral Diarrhea Virus (BVDV)]
Gender female
Storage Conditions liquid nitrogen vapor phase
Karyotype normal female; diploid; stable
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Derivation
CPAE is an endothelial cell line derived from the main stem pulmonary artery of a young cow (Bos taurus). This line was initiated in January, 1979 by P. Del Vecchio from artery lumen scrapings dispersed by enzyme treatment.
Clinical Data
female
Genes Expressed
positive for angiotensin converting enzyme
Comments
Subsequent to the publication of the sixth edition of the ATCC catalog, tests for bovine diarrhea virus (BVD) have indicated that CPAE cells test positive for BVD viral antigen.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM  EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and  observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.  Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week


Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Atmosphere: 95% air; 5% CO2
Name of Depositor PJ Del Vecchio
Year of Origin 1979
References

Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438

Girard JP, Springer TA. Modulation of endothelial cell adhesion by hevin, an acidic protein associated with high endothelial venules. J. Biol. Chem. 271: 4511-4517, 1996. PubMed: 8626806

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Basic Documentation
Other Documentation
References

Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438

Girard JP, Springer TA. Modulation of endothelial cell adhesion by hevin, an acidic protein associated with high endothelial venules. J. Biol. Chem. 271: 4511-4517, 1996. PubMed: 8626806