J27-neo (ATCC® CRL-2372)

Organism: Mus musculus, mouse  /  Cell Type: fibroblast  / 

Organism Mus musculus, mouse
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Strain C3H
Applications
J27-neo is a stable transfected cell line established in 1987 by transfection by calcium phosphate of the J27.2 cell line with a modified neomycin drug-resistant eukaryotic vector, pSP65-Neo.
J27-neo does not secrete or express HLA B7.
Derivation
J27-neo is a stable transfected cell line established in 1987 by transfection by calcium phosphate of the J27.2 cell line with a modified neomycin drug-resistant eukaryotic vector, pSP65-Neo. The cells were selected in HAT and G418.
Comments
J27-neo is a stable transfected cell line established in 1987 by transfection by calcium phosphate of the J27.2 cell line with a modified neomycin drug-resistant eukaryotic vector, pSP65-Neo. The cells were selected in HAT and G418.
The vector did not carry an insert.
J27-neo does not secrete or express HLA B7.
J27.2 is a C3H mouse fibroblast L cell line stably expressing the tk and human b2m genomic genes.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor F Grumet
Year of Origin 1987
References

Kavathas P, Herzenberg LA. Stable transformation of mouse L cells for human membrane T-cell differentiation antigens, HLA and beta 2-microglobulin: selection by fluorescence-activated cell sorting. Proc. Natl. Acad. Sci. USA 80: 524-528, 1983. PubMed: 6188154

Hiraki DD, et al. Bioengineered soluble HLA-B7. Genesis, characterization, and occurrence of dimerization. Hum. Immunol. 40: 235-246, 1994. PubMed: 7960968

Cohen N, et al. Secretion of genetically engineered human/mouse class I antigens. Hum. Immunol. 25: 207-222, 1989. PubMed: 2670852

Basic Documentation
References

Kavathas P, Herzenberg LA. Stable transformation of mouse L cells for human membrane T-cell differentiation antigens, HLA and beta 2-microglobulin: selection by fluorescence-activated cell sorting. Proc. Natl. Acad. Sci. USA 80: 524-528, 1983. PubMed: 6188154

Hiraki DD, et al. Bioengineered soluble HLA-B7. Genesis, characterization, and occurrence of dimerization. Hum. Immunol. 40: 235-246, 1994. PubMed: 7960968

Cohen N, et al. Secretion of genetically engineered human/mouse class I antigens. Hum. Immunol. 25: 207-222, 1989. PubMed: 2670852