L-M (ATCC® CCL-1.2)

Organism: Mus musculus, mouse  / 

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Organism Mus musculus, mouse
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 100 days
Gender male
Strain C3H/An
Applications
Tested and found negative for ectromelia virus (mousepox).
Clinical Data
male
Antigen Expression
H-2k
Genes Expressed
H-2k
Virus Resistance
poliovirus 1, 2, 3
Comments
Grows in protein free medium.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Medium 199 with Earle's BSS supplemented with 0.5% Bacto-peptone
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells from the floor of the flask, aspirate and dispense into new flasks.
Culture Conditions
Temperature: 37.0°C
Name of Depositor DJ Merchant
References

Kuchler RJ, Merchant DJ. Propagation of strain L (Earle) cells in agitated fluid suspension cultures. Proc. Soc. Exp. Biol. Med. 92: 803-806, 1956. PubMed: 13370534

Munyon WH, Merchant DJ. The relation between glucose utilization, lactic acid production and utilization and the growth cycle of L strain fibroblasts. Exp. Cell Res. 17: 490-498, 1959. PubMed: 13672205

Merchant DJ, Hellman KB. Growth of L-M strain mouse cells in a chemically defined medium. Proc. Soc. Exp. Biol. Med. 110: 194-198, 1962. PubMed: 14472756

Hsu TC, Merchant DJ. Mammalian chromosomes in vitro. XIV. Genotypic replacement in cell populations. J. Natl. Cancer Inst. 26: 1075-1083, 1961. PubMed: 13716385

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Kuchler RJ, Merchant DJ. Propagation of strain L (Earle) cells in agitated fluid suspension cultures. Proc. Soc. Exp. Biol. Med. 92: 803-806, 1956. PubMed: 13370534

Munyon WH, Merchant DJ. The relation between glucose utilization, lactic acid production and utilization and the growth cycle of L strain fibroblasts. Exp. Cell Res. 17: 490-498, 1959. PubMed: 13672205

Merchant DJ, Hellman KB. Growth of L-M strain mouse cells in a chemically defined medium. Proc. Soc. Exp. Biol. Med. 110: 194-198, 1962. PubMed: 14472756

Hsu TC, Merchant DJ. Mammalian chromosomes in vitro. XIV. Genotypic replacement in cell populations. J. Natl. Cancer Inst. 26: 1075-1083, 1961. PubMed: 13716385