IRR-MRC-5 [irradiated MRC-5] (ATCC® 55-X™)

Organism: Homo sapiens, human  /  Tissue: lung  /  Cell Type: fibroblast

Organism Homo sapiens, human
Tissue lung
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Age 14 weeks gestation
Gender male
Ethnicity Caucasian
Applications
These cells are provided to be used as feeder cells to support the growth of other cells.
Clinical Data
14 weeks gestation
Caucasian
male
Comments
The cells have been irradiated and will not replicate.
It is recommended that the feeder cells be plated 24 hours before use.
Once the feeder cells have attached, the culture medium may be changed to accommodate the cells to be supported.
These cells will begin to deteriorate in 2 to 3 weeks after plating.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
Name of Depositor JP Jacobs