Clostridium difficile (Hall and O'Toole) Prevot (ATCC® 43255-FZ)

Strain Designations: VPI 10463  /  Biosafety Level: 2

Strain Designations VPI 10463
Application Enteric Research
Isolation Abdominal wound
Biosafety Level 2
Product Format frozen
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Genotype Toxinotype: 0
Images ATCC 43255 Photomicrograph
Comments
Presence of tcdA and tcdB genes confirmed by PCR.
Toxinotype 0
Binary toxin gene cdtB not amplified by PCR
Ribotype 087
Toxin B production confirmed by cytotoxicity assay using Vero cells (Cell line # CCL-81).
Medium ATCC® Medium 5608:
ATCC® Medium 2107: Modified Reinforced Clostridial Agar/Broth Medium (pre-reduced)
Growth Conditions Temperature: 37°C
Atmosphere: Anaerobic gas mixture, 80% N2-10% CO2-10% H2  or 80% N2-20% CO2
Genotype Toxinotype: 0
Name of Depositor TD Wilkins, DM Lyerly
Chain of Custody ATCC <-- TD Wilkins, DM Lyerly <-- G Nygaard
Isolation Abdominal wound
References

Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303

BD GeneOhm Cdiff. BD Becton Dickinson.

Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999

Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986

Kink JA, Williams JA. Antibodies to recombinant Clostridium difficile toxins A and B are an effective treatment and prevent relapse of C. difficile-associated disease in a hamster model of infection. Infect. Immun. 66: 2018-2025, 1998. PubMed: 9573084