Pseudomonas aeruginosa (Schroeter) Migula (ATCC® 27853)

Strain Designations: Boston 41501  /  Type Strain: no  /  Biosafety Level: 2

Strain Designations Boston 41501
Application
Control strain
Food testing
Opportunistic pathogen research
Evaluation of Mueller-Hinton agar Ref Protocols for Evaluating Dehydrated Mueller-Hinton Agar; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M6-A2. RefUS General Services Administration Mueller Hinton Medium, dehydrated. Washington, DC:US General Services Administration;Commercial Item Description A-A-50904A, 1986
Media testing Ref Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M22-A3. Ref Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11133-2:2003. Ref Medical microbiology-- Culture media -- Part 3: Dip slides for microbiological urine analysis. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58942-3: 2003, 2003 Ref Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution;British Standard DD CEN ISO/TS 11133:2003.
Quality control strain Ref API 20 NE. bioMerieux. Ref BD Enterotube II QC Set. BD Becton Dickinson; Enterotube  Ref BD Oxi/Ferm II QC Set. BD Becton Dickinson; Oxi/Ferm  RefBD Phoenix Gram Negative AST NMIC/ID QC Set. BD Becton Dickinson; Phoenix  Ref MicroScan Dried Gram Negative Panel QC Set. Dade/Siemens. Ref Sensititre™ Automated Gram Positive ID. Thermo Scientific™ Sensititre™ Systems, Trek Diagnostic Systems. Ref Sensititre™ MIC Susceptibility. Thermo Scientific™ Sensititre™ Systems, Trek Diagnostic Systems. ref
Susceptibility disc testing Ref Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard - 9th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M2-A9. Ref Methods for Antimicrobial Disk Susceptibility Testing of Bacteria Isolated from Aquatic Animals. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M42-A. Ref Methods for Broth Dilution Susceptibility Testing of Bacteria Isolated from Aquatic Animals. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M49-A.
Susceptibility testing Ref Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard - 7th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M7-A8. Ref Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard - 2nd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M31-A3. Ref Performance Standards for Antimicrobial Susceptibility Testing; Sixteenth Informational Supplement. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M100-S20, 2007 Ref Evaluation of Lots of Dehydrated Mueller-Hinton Broth for Antimicrobial Susceptibility testing; Proposed Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M32-P. RefMedical microbiology; methods for the determination of susceptibility of pathogens (except mycobacteria) to antimicrobial agents: agar diffusion test. Berlin, Germany: Deutsches Institut fur Normung; DIN 58940-3: 1989 Ref Medical microbiology -- Methods for the determination of susceptibility of pathogens (except mycobacteria) to antimicrobial agents-- Part 7: Determination of the minimum bactericidal concentration (MBC) with the aid of microdilution. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58940-7: 1994, 1994 Ref Medical microbiology: methods for the determination of susceptibility of pathogens (except mycobacteria) to antimicrobial agents; determination of MICs by agar dilution method. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58940-6: 1989, 1989 RefMedical microbiology -- Susceptibility testing of microbial pathogens to antimicrobial agents -- Part 81: Microdilution; Special requirements for testing of non-fastidious bacteria. Berlin, Germany: Deutsches Institut fur Normung; DIN 58940-81: 2002 Ref Clinical laboratory testing and in vitro diagnostic test systems --- Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices --- Part 1: Reference method for testing the in vitro activity of antimicrobial agents against rapidly growing aerobic bacteria involved in infectious diseases. London, UK:British Standards Institution;British Standard BS EN ISO 20776-1:2006.
Testing Ref Aeromonas in Finished Water by Membrane Filtration using Ampicillin-Dextrin Agar with Vancomycin (ADA-V). Washington, DC:Environmental Protection Agency;EPA EPA Method 1605, 2001
Quality control strain for Abbott, API, Autobac, BBL, bioMerieux Vitek, Micro-Media, MicroScan®, Roche Diagnostics, Sensititre products, and the Etest Metallo Beta-Lactamase (MBL) strip.
Isolation
Blood culture
Type Strain no
Biosafety Level 2
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® yes
Type Strain no
Comments
Incorrectly cited in previous editions of this catalog as identical to NCTC 10662.
effect of salt concentration on gentamicin susceptibility
Medium ATCC® Medium 18: Trypticase Soy Agar/Broth
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor AA Medeiros
Isolation
Blood culture
References

Medeiros AA, et al. Effect of salt concentration on the apparent in-vitro susceptibility of Pseudomonas and other gram-negative bacilli to gentamicin. J. Infect. Dis. 124: 59-66, 1971. PubMed: 5001630

Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard - 9th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M2-A9.

Protocols for Evaluating Dehydrated Mueller-Hinton Agar; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M6-A2.

Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard - 7th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M7-A8.

Methodology for the Serum Bactericidal test: Approved Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M21-A.

Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M22-A3.

Methods for Determining Bactericidal Activity of Antimicrobial Agents; Approved Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M26-A.

Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard - 2nd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M31-A3.

US General Services Administration Mueller Hinton Medium, dehydrated. Washington, DC:US General Services Administration;Commercial Item Description A-A-50904A, 1986

Hyland SA, et al. Cloning, expression, and purification of UDP-3-O-acyl-GlcNAc deacetylase from Pseudomonas aeruginosa: a metalloamidase of the lipid A biosynthesis pathway. J. Bacteriol. 179: 2029-2037, 1997. PubMed: 9068651

O'Callaghan RJ, et al. The role of an uncharacterized exoprotein, protease IV, in corneal virulence. Invest. Ophthalmol. Vis. Sci. 37: 534-543, 1996. PubMed: 8595953

Performance Standards for Antimicrobial Susceptibility Testing; Sixteenth Informational Supplement. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M100-S20, 2007

API 20 NE. bioMerieux.

BD Enterotube II QC Set. BD Becton Dickinson; Enterotube

BD Oxi/Ferm II QC Set. BD Becton Dickinson; Oxi/Ferm

BD Phoenix Gram Negative AST NMIC/ID QC Set. BD Becton Dickinson; Phoenix

Colilert. IDEXX.

Colisure. IDEXX.

MicroScan Dried Gram Negative Panel QC Set. Dade/Siemens.

Sensititre™ Automated Gram Positive ID. Thermo Scientific™ Sensititre™ Systems, Trek Diagnostic Systems.

Sensititre™ MIC Susceptibility. Thermo Scientific™ Sensititre™ Systems, Trek Diagnostic Systems.

Methods for Antimicrobial Disk Susceptibility Testing of Bacteria Isolated from Aquatic Animals. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M42-A.

Listeria species -- Biochemical Identification Method (Vitek GPI and GNI+). Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 992.19.

Evaluation of Lots of Dehydrated Mueller-Hinton Broth for Antimicrobial Susceptibility testing; Proposed Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M32-P.

Methods for Broth Dilution Susceptibility Testing of Bacteria Isolated from Aquatic Animals. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M49-A.

Aeromonas in Finished Water by Membrane Filtration using Ampicillin-Dextrin Agar with Vancomycin (ADA-V). Washington, DC:Environmental Protection Agency;EPA EPA Method 1605, 2001

Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11133-2:2003.

Medical microbiology; methods for the determination of susceptibility of pathogens (except mycobacteria) to antimicrobial agents: agar diffusion test. Berlin, Germany: Deutsches Institut fur Normung; DIN 58940-3: 1989

Medical microbiology -- Methods for the determination of susceptibility of pathogens (except mycobacteria) to antimicrobial agents-- Part 7: Determination of the minimum bactericidal concentration (MBC) with the aid of microdilution. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58940-7: 1994, 1994

Medical microbiology: methods for the determination of susceptibility of pathogens (except mycobacteria) to antimicrobial agents; determination of MICs by agar dilution method. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58940-6: 1989, 1989

Medical microbiology -- Susceptibility testing of microbial pathogens to antimicrobial agents -- Part 81: Microdilution; Special requirements for testing of non-fastidious bacteria. Berlin, Germany: Deutsches Institut fur Normung; DIN 58940-81: 2002

Medical microbiology-- Culture media -- Part 3: Dip slides for microbiological urine analysis. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58942-3: 2003, 2003

Clinical laboratory testing and in vitro diagnostic test systems --- Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices --- Part 1: Reference method for testing the in vitro activity of antimicrobial agents against rapidly growing aerobic bacteria involved in infectious diseases. London, UK:British Standards Institution;British Standard BS EN ISO 20776-1:2006.

Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution;British Standard DD CEN ISO/TS 11133:2003.

Etest Metallo Beta-Lactamase strip

Colilert-18 QC Set. IDEXX.

VITEK 2 AST-GN QC Set. bioMerieux.

RapID™ ONE QC Set. Remel™.

MicroScan ESBL plus QC Set. Dade/Siemens.

MicroScan Rapid Neg ID Type 4 QC Set. Dade/Siemens.

MicroScan Streamlined ID QC Set. Dade/Siemens.

MicroScan Synergies plus Gram Negative QC Set. Dade/Siemens.

ISO/CD 11133:2009, Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions)

Corry, JEL, Curtis, GDW and Baird, RM (Eds) Handbook of Culture Media for Food and Water Microbiology. Royal Society of Chemistry, In preparation. ICFMH-WPCM.

ETEST ESBL STRIP. bioMerieux.

ETEST MBL STRIP. bioMerieux.

Pseudalert QC Set. IDEXX.

Sensititre™ MIC Breakpoint QC Set/5. Thermo Scientific™ Sensititre™ Systems, Trek Diagnostic Systems.

Cross References

Nucleotide (GenBank) : X65933 P. aeruginosa pa-1L gene for PA-I galactophilic lectin.

Nucleotide (GenBank) : U07832 Pseudomonas aeruginosa helicase I (helA) gene, partial cds.

Nucleotide (GenBank) : U64101 Pseudomonas aeruginosa heat shock protein (groesEL) gene, partial cds.

Nucleotide (GenBank) : AB037545 Pseudomonas aeruginosa gene for 16S ribosomal RNA, strain:ATCC 27853.

Nucleotide (GenBank) : M63957 Pseudomonas aeruginosa heat shock protein (groES and groEL) genes, complete cds.

Nucleotide (GenBank) : U67855 Pseudomonas aeruginosa UDP-3-O-acyl-GlcNAc deacetylase (lpxC) gene, complete cds.

Nucleotide (GenBank) : AF094719 Pseudomonas aeruginosa strain ATCC 27853 16S ribosomal RNA gene, partial sequence.