Rhizopus oryzae Went et Prinsen Geerligs, teleomorph (ATCC® 9363)

Strain Designations: NRRL 395 [IMI 40564]  /  Product Format: freeze-dried

Permits and Restrictions

View Permits

Strain Designations NRRL 395 [IMI 40564]
degrades barley
degrades cassava
degrades corn
degrades rice
degrades sugarcane bagasse
produces L(+)-lactic acid
produces NAD-dependent lactate dehydrogenase
produces amylase
produces chitosan
produces ergostadientriols
produces fumaric acid
produces lactic acid lactate
produces ergostadientriols useful in lowering serum cholesterol levels
produces L(+)-lactic acid from ground corn
produces L(+)-lactic acid from barley, cassava, corn, oats and rice
produces L(+)-lactic acid in calcium alginate gels
produces L(+)-lactic acid from sugarcane bagasse
produces amylolytic enzymes
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Type Strain no
Preceptrol® no
source of lactate dehydrogenase genes used in the construction of expression vectors
Tempeh fermentation
Medium ATCC® Medium 336: Potato dextrose agar (PDA)
Growth Conditions
Temperature: 24.0°C
Sequenced Data
No DNA sequencing was performed in house on this product.
Name of Depositor NRRL
source of lactate dehydrogenase genes used in the construction of expression vectors
Cross References

Nucleotide (GenBank) : AF226154 lactate dehydrogenase (ldhA) gene, complete coding sequence

Nucleotide (GenBank) : AF226155 lactate dehydrogenase (ldhB) gene, complete coding sequence


Goldberg I, Stieglitz B. Improved rate of fumaric acid production by Tweens and vegetable oils in Rhizopus arrhizus. Biotechnol. Bioeng. 27: 1067-1069, 1985.

Zilliken FW. Ergostadienetriols, compositions containing same, and methods of preparing and using same. US Patent 4,234,577 dated Nov 18 1980

. . Appl. Microbiol. Biotechnol. 41: 286-290, 1994.

Yu RC, Hang YD. Purification and characterization of NAD-dependent lactate dehydrogenase from Rhizopus oryzae. Food Chem. 41: 219-225, 1991.

Skory CD. Isolation and expression of lactate dehydrogenase genes from Rhizopus oryzae. Appl. Environ. Microbiol. 66: 2343-2348, 2000. PubMed: 10831409

Skory CD. Fungal lactate dehydrogenase gene and constructs for the expression thereof. US Patent 6,368,189 dated Jul 31 2001

Hang YD. Direct fermentation of corn to L(+)-lactic acid by Rhizopus oryzae. Biotechnol. Lett. 11: 299-300, 1989.

Hang YD, et al. Production of L(+)-lactic acid by Rhizopus oryzae immobilized in calcium alginate gels. Biotechnol. Lett. 11: 119-120, 1989.

Yu RC, Hang YD. Kinetics of direct fermentation of agricultural commodities to L(+)-lactic acid by Rhizopus oryzae. Biotechnol. Lett. 11: 597-600, 1989.

Yu RC, Hang YD. Amylolytic enzyme production by Rhizopus oryzae grown on agricultural commodities. World J. Microbiol. Biotechnol. 6: 15-18, 1990.

Hang YD. Chitosan production from Rhizopus oryzae mycelia. Biotechnol. Lett. 12: 911-912, 1990.

Soccol CR, et al. Production of L-lactic acid by Rhizopus species. World J. Microbiol. Biotechnol. 10: 433-435, 1994.

Pritchard GG. Factors affecting the activity and synthesis of NAD-dependent lactate dehydrogenase in Rhizopus oryzae. J. Gen. Microbiol. 78: 125-137, 1973.

Ward GE, et al. Biochemical studies in the genus Rhizopus. I. The production of dextro-lactic acid. J. Am. Chem. Soc. 58: 1286-1288, 1936.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation