Aspergillus oryzae (Ahlburg) Cohn, anamorph (ATCC® 20386)

Strain Designations: 460 [FERM-BP 983, FERM-P 1149]  /  Product Format: freeze-dried

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Strain Designations 460 [FERM-BP 983, FERM-P 1149]
produces acid carboxypeptidase
produces acid protease protease (acid)
produces alkaline protease
produces amylase, alpha 1A; salivary Amylase, salivary, alpha-1A
produces carboxymethyl cellulase CM-celluase
produces cytosol aminopeptidase leucine aminopeptidase
produces pectin lyase pectic acid trans-eliminase
produces peptidase
produces protease nonstructural protein 3
produces soy sauce
produces protease inhibitor kojistatin A
Biosafety Level 1
Product Format freeze-dried
Type Strain no
Preceptrol® no
Medium ATCC® Medium 323: Malt agar medium
Growth Conditions
Temperature: 30.0°C
Sequenced Data
Not available.
Name of Depositor Noda Inst. Sci. Res.
Cross References

Nucleotide (GenBank) : E02376 Preproalkaline protease gene.

Nucleotide (GenBank) : E02318 DNA encoding alkaline protease.

Nucleotide (GenBank) : E05048 DNA encoding neutral protease II.

Nucleotide (GenBank) : E03070 cDNA sequence coding for neutral protease II.

Nucleotide (GenBank) : E03071 cDNA sequence coding for pre-,pro-,neutral protease II.


Nakadai T, Nasuno S. Enzyme preparation from extract of wheat bran koji by alcohol precipitation. J. Ferment. Bioeng. 67: 253-257, 1989.

Nakadai T, Nasuno S. Use of glutaminase for soy sauce made by koji or a preparation of proteases from Aspergillus oryzae. J. Ferment. Bioeng. 67: 158-162, 1989.

Nakadai T, et al. Process for manufacturing soy sauce using enzymatic preparation(s). US Patent 3,914,436 dated Oct 21 1975

Ida Y, et al. Kojistatin A, a new cysteine protease inhibitor produced by Aspergillus oryzae. Biosci. Biotechnol. Biochem. 60: 1747-1748, 1996.

Nakadai T, Nasuno S. Enzymic hydrolysis of protein by various enzyme preparations. J. Ferment. Technol. 54: 872-884, 1976.

Nakadai T, Nasuno S. Hyperproduction of peptidase and proteinase by mutants of Aspergillus oryzae. J. Ferment. Technol. 55: 273-276, 1977.

Nakadai T, Nasuno S. The action of acid proteinase from Aspergillus oryzae on soybean proteins. Agric. Biol. Chem. 41: 409-410, 1977.

Kakadai T, Nasuno S. Culture conditions of Aspergillus oryzae for production of enzyme preparation. J. Ferment. Technol. 66: 525-533, 1988.

Tatsumi H, et al. Cloning and sequencing of the alkaline protease cDNA from Aspergillus oryzae. Agric. Biol. Chem. 52: 1887-1888, 1988.

Tatsumi H, et al. Autoproteolytic processing and processing-dependent secretion of Aspergillus oryzae alkaline protease in yeast. Agric. Biol. Chem. 55: 3099-3101, 1991.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation