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Karyotype
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Number of cells examined = 80; Modal Chromosome Number = 79 with a range of 65 to 84; Polyploidy Rate = 14.3%
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Derivation
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The line was established from cells in the pleural fluid of a patient with small cell carcinoma of the lung. The patient had previously been treated with cytoxan, vincristine, methotrexate and radiation therapy.
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Receptor Expression
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epidermal growth factor (EGF)
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Oncogene
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c-myc +; N-myc +; c-raf-1 +; H-ras +; K-ras +; N-ras +; v-fes -; v-fms -
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Genes Expressed
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adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; calcitonin; corticotropin; beta endorphin; 17 beta estradiol; lipotropin; oxytocin - neurophysin (OT-NP); parathormone; somatostatin-like immunoreactivity (SRIF),c-myc +; N-myc +; c-raf-1 +; H-ras +; K-ras +; N-ras +; v-fes -; v-fms -,Leu 7; My23
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Comments
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The cell express HLA class I and class II antigens.
Derivative chromosomes observed at high frequency in 24 karyotyped metaphases included: der(2)t(2;?18)(q10;q10); del(3)?(pter-->p21::q10-->qter); del(6)(q13); der(10); add(11)(p15); der(14)t(14;17)(q10;q10); add(15)(p11); add(15)(p11)+; and del(17)(?q24).
Two copies of each derivative chromosome was observed in each metaphase.
Less frequent derivative chromosomes included: del(12)?(q11q21)[18/24 cells] and add(13)(p11)[10/24 cells]. Four to eight additional marker chromosomes of unknown origin were generally detected in each cell.
Cytogenetics Comments: This is a hyper-triploid human cancer cell line with a modal chromosome number of 79.
A Y chromosome was verified by QM staining and C-banding. Normal chromosomes 10, 15 and 17 were absent.
The composite karyotype consists of 65 to 84, XY, +1, -2, der(2)t(2;?18)(q10;q10)(x2); -3, del(3)?(pter-->p21::q10-->qter)x2, -4, -5, +6, del(6)(?q15)x2,+7,+8,-9,-10,-10,-10, der(10)x2, -11, add(11)(p15)x2, -12, del(12)?(q11q21)[18], -13, +14, der(14)t(14;17)(q10;q10)x2, -15, -15,- 15, der(15)x2, -16, -17, -17, 17, del(17)x2, -18, +19, -20, +21, -22[cp24]
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