Escherichia coli (Migula) Castellani and Chalmers (ATCC® 700927)

Strain Designations: EDL 933  /  Type Strain: no  /  Biosafety Level: 2

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Classification Enterobacteriaceae, Escherichia
Strain Designations EDL 933
Application
Enteric Research
Isolation
Derived from existing strain CDC EDL 933, ATCC 43895
Type Strain no
Biosafety Level 2
Antigenic Properties Serotype O157:H7:K- RefPerna NT, et al. Molecular evolution of a pathogenicity island from enterohemorrhagic Escherichia coli O157:H7. Infect. Immun. 66: 3810-3817, 1998. PubMed: 9673266, RefPlunkett G, et al. Sequence of Shiga toxin 2 phage 933W from Escherichia coli O157:H7: Shiga toxin as a phage late-gene product. J. Bacteriol. 181: 1767-1778, 1999. PubMed: 10074068, RefBurland V, et al. The complete DNA sequence and analysis of the large virulence plasmid of Escherichia coli O157:H7. Nucleic Acids Res. 26: 4196-4204, 1998. PubMed: 9722640, (Guy Plunkett, personal communication)
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Genome Sequenced Strain

Escherichia coli O157:H7 EDL933, complete genome

Type Strain no
Antigenic Properties Serotype O157:H7:K- RefPerna NT, et al. Molecular evolution of a pathogenicity island from enterohemorrhagic Escherichia coli O157:H7. Infect. Immun. 66: 3810-3817, 1998. PubMed: 9673266, RefPlunkett G, et al. Sequence of Shiga toxin 2 phage 933W from Escherichia coli O157:H7: Shiga toxin as a phage late-gene product. J. Bacteriol. 181: 1767-1778, 1999. PubMed: 10074068, RefBurland V, et al. The complete DNA sequence and analysis of the large virulence plasmid of Escherichia coli O157:H7. Nucleic Acids Res. 26: 4196-4204, 1998. PubMed: 9722640, (Guy Plunkett, personal communication)
Comments
University of Wisconsin genome project strain
Genome sequenced strain
Medium Medium 3: Nutrient agar or nutrient broth
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor G Plunkett
Special Collection NCRR Contract
Chain of Custody
ATCC <-- G Plunkett <-- C. Kaspar
Isolation
Derived from existing strain CDC EDL 933, ATCC 43895
References

Perna NT, et al. Molecular evolution of a pathogenicity island from enterohemorrhagic Escherichia coli O157:H7. Infect. Immun. 66: 3810-3817, 1998. PubMed: 9673266

Plunkett G, et al. Sequence of Shiga toxin 2 phage 933W from Escherichia coli O157:H7: Shiga toxin as a phage late-gene product. J. Bacteriol. 181: 1767-1778, 1999. PubMed: 10074068

Burland V, et al. The complete DNA sequence and analysis of the large virulence plasmid of Escherichia coli O157:H7. Nucleic Acids Res. 26: 4196-4204, 1998. PubMed: 9722640

Perna NT, et al. Genome sequence of enterohemorrhagic Escherichia coli O157:H7. Nature 409: 529-533, 2001. PubMed: 11206551

Guy Plunkett, personal communication

Perna NT, et al. Molecular evolution of a pathogenicity island from enterohemorrhagic Escherichia coli O157:H7. Infect. Immun. 66: 3810-3817, 1998. PubMed: 9673266

Plunkett G, et al. Sequence of Shiga toxin 2 phage 933W from Escherichia coli O157:H7: Shiga toxin as a phage late-gene product. J. Bacteriol. 181: 1767-1778, 1999. PubMed: 10074068

Burland V, et al. The complete DNA sequence and analysis of the large virulence plasmid of Escherichia coli O157:H7. Nucleic Acids Res. 26: 4196-4204, 1998. PubMed: 9722640

Guy Plunkett, personal communication

Perna NT, et al. Molecular evolution of a pathogenicity island from enterohemorrhagic Escherichia coli O157:H7. Infect. Immun. 66: 3810-3817, 1998. PubMed: 9673266

Plunkett G, et al. Sequence of Shiga toxin 2 phage 933W from Escherichia coli O157:H7: Shiga toxin as a phage late-gene product. J. Bacteriol. 181: 1767-1778, 1999. PubMed: 10074068

Burland V, et al. The complete DNA sequence and analysis of the large virulence plasmid of Escherichia coli O157:H7. Nucleic Acids Res. 26: 4196-4204, 1998. PubMed: 9722640

Guy Plunkett, personal communication

Cross References

Nucleotide (GenBank) : AF071034 LEE element sequence

Nucleotide (GenBank) : AF074613 pO157 plasmid sequence

Nucleotide (GenBank) : AF125520 933W bacteriophage sequence

Nucleotide (GenBank) : AE005174 Escherichia coli O157:H7 EDL933, complete genome

Nucleotide (GenBank) : DQ272420.1 Escherichia coli strain ATCC 700927 clone 2 putative outer membrane lipoprotein (nlpD) gene, partial cds; and truncated sigma 38 factor (rpoS) gene, complete cds

Nucleotide (GenBank) : DQ272419.1 Escherichia coli strain ATCC 700927 clone 1 putative outer membrane lipoprotein (nlpD) gene, partial cds; and truncated sigma 38 factor (rpoS) gene, complete cds

Nucleotide (GenBank) : CP008958.1 Escherichia coli O157:H7 str. EDL933 plasmid, complete sequence

Nucleotide (GenBank) : CP008957.1 Escherichia coli O157:H7 str. EDL933, complete genome

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These permits may be required for shipping this product:

  • This item is listed on the Commerce Control List of the U.S. Department of Commerce and is distributed only within the 50 United States. Distribution requires completion of a Customer Acceptance of Responsibility (CAR) for Commerce Control List Biologicals form.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation