Candida parapolymorpha Suh et Zhou (ATCC® 26012)

Strain Designations: DL-1 [NRRL Y-7560]  /  Product Format: frozen

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Deposited As Hansenula polymorpha de Morais et Maia, teleomorph
Strain Designations DL-1 [NRRL Y-7560]
Application
produces 2-butanone alcohol dehydrogenase
produces formaldehyde
produces hydrogen peroxide
produces isopropanol dehydrogenase (NADP) secondary alcohol dehydrogenase
produces methyl ketones
transformation host
utilizes methanol
reduces 4-chloro-1-(4-fluorophenyl)butan-1-one
transformation host for expression of hirudin gene
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain yes
Preceptrol® no
Genome Sequenced Strain

Yes

Comments
Mitochondrial genome sequenced strain. RefEldarov MA, et al. Complete sequence and analysis of the mitochondrial genome of the methylotrophic yeast Hansenula polymorpha DL-1. FEMS Yeast Res. 11(6):464-472, 2011. PubMed: 21545683.
Genome sequencing strain (the Joint Genome Institute at the Department of Energy, USA; Korea Research Institute of Bioscience and Biotechnology, S. Korea; Centre 'Bioengineering' RAS, Russia).
Medium ATCC® Medium 28: Emmons' modification of Sabouraud's agar
ATCC® Medium 200: YM agar or YM broth
ATCC® Medium 1245: YEPD
Growth Conditions
Temperature: 24°C to 26°C
Atmosphere: Typical aerobic
Sequenced Data
SSU rDNA of ATCC 26012

AAAGATTAAGCCATGCATGTCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAGTTCCTTTACTACATGGATAACCGTGGTAATTCTAGAGCTAATACATGCTTAAAGCCCCGACTTCTGGAAGGGGTGTATTTATTAGATAAAAAATCAATGCCTTCGGGCTTTTTGATGATTCATAATAACTTTTCGAAGCTCATGGCCTTGTGCTGGAGCTGGTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCCTACCATGGTTTTCACGGGTAACGGGGAATAAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACAGGGAGGTAGTGACAATATATAACGATACAGGGCCCTTTCGGGTCTTGTAATTGGAATGAGTACAATGTAAATACCTTAACGAGGAACAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGTCTGGTTAGCCGGTCTGCCTTTTTTGGCACGTACTGGATTTAACCGGGCCTTTCCTTCTGGCTAACCAACACTCTGTGTTGGCAAACCAGGACTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCTTTGCTCGAATATATTAGCATGGAATAATAGAATAGGACGTTATGGTTCTATTTTGTTGGTTTCTAGGACCATCGTAATGATTAATAGGGACGGTCGGGGGCATCAGTATTCAGTTGTCAGAGGTGAAATTCTTGGATTTACTGAAGACTAACTACTGCGAAAGCATTTGCCAAGGACGTTTTCATTAATCAAGAACGAAAGTTAGGGGATCGAAGATGATCAGATACCGTCGTAGTCTTAACCATAAACTATGCCGACTAGGGATCGGGTGGTGTTTCTTTTTTGACCCACTCGGCACCTTACGAGAAATCAAAGTTTTTGGGTTCTGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATTTTGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTTAACCTACTAAATAGTGATATTGGCTTTTGCTGATTTTCACTTCTTAGAGGGACTATCGGTTTCAAGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGACGTTCTGGGCCGCACGCGCGCTACACTGACGGAGCCAACGAGCTCTATCCTTGACCGAGAGGTCCGGGTAATCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGCAATTATTGCTCTTCAACGAGGAATTCCTAGTAAGCGCAAGTCATCAGCTTGCGTTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTAGTACCGATTGAATGGCTTAGTGAGGCTTCAGGATTGGTTCAGAGAAGGGGGCAACTCCATCTTGGAACCGAGAATCTAGTCAAACTTGGTCATTTAGAGGAACTAAAA


ITS of ATCC 26012

TTTCCGTAGGTGAACCTGCGGAAGGATCATTACAGTATTCTTCTAAGCTGGAGCTGTTAATTGATTGAACTGTTTGCCTGCGCTGCTGAAATACTGCGTGCGCGGTGATGGTTTTTTTGGTTGCAGTTCTACTCAAGGCTTAGAAATCTCCTTACACACTTTGTTTTCTTATTAGAGACAAATTTGCTTTGGCTAGCGGTCGTTTAAACGCGGCATGCCTGGCCAGAGGTTTTAACAAAACTCATTTTCGTCTTGCCCTCGTGGGAAGATTGAAAACTTGTATTATTATTTCGTCAGTTATAAGAAGTTGCGTTTGCAGCTTTTCTAAACTTTAAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGATTTTCGTGAATCATTGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCAGAGGGCATGCCTGTTTGAGCGTCATTTCCCTCTCAAACCCCCGGGTTTGGTGATGGGCAATACTCTCGTCTGAGAGTTTGCTTGAAATGAATTGGCATGAGTTTTTGATACGAACTTCAGCTGTTTATTCAATGTATTAGGTTTTATCCATCGTTGATTGACAACTGCAAGCTTCTTTCAAAATTTGGCTCTGCCTTACAAACTTTAATCATAAAGTTTGACCTCAAATCAGGTAGGATTACCCGCTGAACTTAAGCATATCAAT


D1/D2 region of LSU rDNA of ATCC 26012

ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTTAGTAGCGGCGAGTGAAGCGGCAAGAGCTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGAAGAAAGTAATCTTGGAGGTGGCCTTTGTCTATGTTCCTTGGAACAGGACGTCATGGAGGGTGAGAATCCCGTGTGATGAGGTGTCCATCTCCGTGTAAGATGCTTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCAAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACTGTGAAGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAGTACGTGAAATTGTTGAAAGGGAAGGGTATTTGATCAGACTTGGTATTTAGCTATCATCGCTCCTTGTGGGTGGTGCTCTAGCTTTTTACTGGGCCAGCATCAGTTTTGGTGGCAAGATAATGACAGTTGAATGTGGCTCCTCGGAGTGTTATAGCTTCTGTTGATATTGCCTACCGGGACTGAGGTCTGCGGCTTTTGCCTAGGATGCTGGCGTAATGATCCAATACCGCCCGT

Name of Depositor DW Levine
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
Chain of Custody
ATCC <-- DW Levine <-- CL Cooney
Isolation
Soil, Massachusetts
Cross References

Nucleotide (GenBank) : JN863704 RPB 2 gene

Nucleotide (GenBank) : JN863698 RPB 1 gene

Nucleotide (GenBank) : FJ914939 D1/D2 region of 28S rRNA gene

Nucleotide (GenBank) : FJ914922 ITS including 5.8S rRNA gene

Nucleotide (GenBank) : FJ914907 18S rRNA gene

References

Patel RN, et al. Method for producing microbial cells and use thereof to produce oxidation products. US Patent 4,266,034 dated May 5 1981

Patel RN, et al. Microbial reduction of 1-(4-fluorophenyl)-4-[4-(5-fluoro-2-pyrimidinyl)-1-piperazinyl]butan-1-one. Biotechnol. Appl. Biochem. 17: 139-153, 1993. PubMed: 8166884

Baratti J, et al. Preparation and properties of immobilized methanol oxidase. Biotechnol. Bioeng. 20: 333-348, 1978.

Kim CH, et al. Effect of soybean oil on the enhanced expression of hirudin gene in Hansenula polymorpha. Biotechnol. Lett. 18: 417-422, 1996.

Cooney CL, Levine DW. Microbial utilization of methanol. Adv. Appl. Microbiol. 15: 337-365, 1972. PubMed: 4593294

Kang HA, et al. Cloning and characterization of the Hansenula polymorpha homologue of the Saccharomyces cerevisiae PMR1 gene. Yeast 14: 1233-1240, 1998. PubMed: 9791894

Kushnirov VV. Rapid and reliable protein extraction from yeast. Yeast 16: 857-860, 2000. PubMed: 10861908

Bravo M, Wang DI. Enzymatic oxidation of methanol to produce formaldehyde and hydrogen peroxide. Adv. Biotechnol. 3: 329-334, 1981.

Patel RN, et al. Oxidation of secondary alcohols to methyl ketones by yeasts. Appl. Environ. Microbiol. 38: 219-223, 1979. PubMed: 42348

Patel RN, et al. Microbial production of methyl ketones. Purification and properties of a secondary alcohol dehydrogenase from yeast. Eur. J. Biochem. 101: 401-406, 1979. PubMed: 230031

Suh SO, Zhou JJ. Methylotrophic yeasts near Ogataea (Hansenula) polymorpha: a proposal of Ogataea angusta comb. nov. and Candida parapolymorpha sp.nov. FEMS Yeast Res. 10: 631-638, 2010. PubMed: 20491937

Eldarov MA, et al. Complete sequence and analysis of the mitochondrial genome of the methylotrophic yeast Hansenula polymorpha DL-1. FEMS Yeast Res. 11(6):464-472, 2011. PubMed: 21545683.

Ravin NV, et al. Genome sequence and analysis of methylotrophic yeast Hansenula polymorpha DL1. BMC Genomics 14: 837, 2013. PubMed: 24279325

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Basic Documentation