Zygosaccharomyces rouxii (Boutroux) Yarrow, teleomorph (ATCC® 14679)

Alternate State: Candida mogii Vidal-Leiria, anamorph, Candida placentae Goto, anamorph  /  Strain Designations: NRRL Y-2547 [CBS 4837, CCRC 21487, DSM 2531, IFO 1876, NCYC 751]  /  Product Format: freeze-dried

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Deposited As Saccharomyces rouxii Boutroux, teleomorph
Strain Designations NRRL Y-2547 [CBS 4837, CCRC 21487, DSM 2531, IFO 1876, NCYC 751]
Alternate State Candida mogii Vidal-Leiria, anamorph, Candida placentae Goto, anamorph
Application
Produces miso
Transformation host
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain no
Preceptrol® no
Mating Type mating type a RefOshio K, et al. Construction of a host-vector system in the osmophilic haploid yeast Zygosaccharomyces rouxii. J. Ferment. Technol. 66: 481-488, 1988.
Comments
Devoid of any plasmids
Miso fermentation
Sequenced Data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence

GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTATAGAAAATGACGTGAACTCTTAACGGAGTTCTCTCAAAGTGTTGGAGGGGAAGGCCTGCGCTTAATTGCGCGGCTGTTTTTAATCTCCTCCGCCTTTGATACACACATTGGAGTTTCTACTTTTTTGTTCTCTTTGGGAGGGTTCTGCTCTCCCAGAGGTAAACACAAACAATCTTTTATTATACTATTAACACAGTCAAATGAATTTTAAAAACAAAATATTCAAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAACTGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTGTTTGAGCGTCATTTCCCTCTCAAACTTTACGTTTGGTAGTGAGCGATACTCTACTCTGGAGTTTGCTTGAAAATGGGAGGCCATAGGCGGAGCTTAGTTTGCGACTGTGCCGAGAGGCATGGGAGCGGCCTAGCCACGAAAAGTCGTATTAGGTTTTACCGACTCGGCGGAATAGTGGAGAGGTTTCTTTTTTTTTTATTCTAAGGTAAGCCGTCTGGCTAGACAAAATTCTCAAAGTTTGACCTCAAATCAGGTAGGATTACCCGCTGAACTTAAGCATATCAATAA


D1D2 region of the 26S ribosomal RNA gene

ATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTACCTTTCGGTGCCCGAGTTGTAATTTGGAGAGAGTGATTCTGGGACTGGCCCTTGCCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAACCCCGTGAGGCGAGGTGATCCAGTTCTTTGTAGAGCGCTCTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGAGGGTGGTAAATTCCATCTAAAGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAGGACGTGAAATTGTTGAAAGGGAAGGGCATTTGATCAGACATGGTGTTTTGTGCCCCTCGCTCCTCGTGGGTGGGGGAATCTCGCAGCTCACTGGGCCAGCATCAGTTTTGGCGGCAGGAGAAATCCTCGGGAATGTGGCTCTTGCCTCTGGTAGGAGTGTTATAGCCCGGGGGAATACTGCCAGCTGGGACTGAGGTATGCGACATTTTGTCAAGGATGTTGGCATAATGGTTATATGCCGC

Name of Depositor NRRL
Isolation
Japanese miso
References

Smith AK, et al. Preparation of miso. US Patent 2,967,108 dated Jan 3 1961

Hesseltine CW. A millennium of fungi, food, and fermentation. Mycologia 57: 149-197, 1965.

Oshio K, et al. Construction of a host-vector system in the osmophilic haploid yeast Zygosaccharomyces rouxii. J. Ferment. Technol. 66: 481-488, 1988.

Solieri L, et al. Unravelling genomic diversity of Zygosaccharomyces rouxii complex with a link to its life cycle. FEMS Yeast Res.13: 245-258, 2013. PubMed: 23279556

Shepherd A, Piper PW. The Fps1p aquaglyceroporin facilitates the use of small aliphatic amides as a nitrogen source by amidase-expressing yeasts. FEMS Yeast Res.10: 527-534, 2010. PubMed: 20491941

Suezawa Y, Suzuki M, Mori H. Genotyping of a miso and soy sauce fermentation yeast, Zygosaccharomyces rouxii, based on sequence analysis of the partial 26S ribosomal RNA gene and two internal transcribed spacers. Biosci. Biotechnol. Biochem. 72: 2452-2455, 2008. PubMed: 18776675

James SA, et al. Molecular evidence for the existence of natural hybrids in the genus Zygosaccharomyces. FEMS Yeast Res. 5: 747-755, 2005. PubMed: 15851103

Oshio K, et al. Construction of a host-vector system in the osmophilic haploid yeast Zygosaccharomyces rouxii. J. Ferment. Technol. 66: 481-488, 1988.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation