Schizochytrium limacinum Honda et Yokochi (ATCC® MYA-1381)

Strain Designations: SR21 [IFO 32693]  /  Product Format: frozen

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Deposited As Schizochytrium limacinum Honda et Yokochi
Classification Chromista
Strain Designations SR21 [IFO 32693]
Application
Produces docosahexaenoic acids
Produces docosapentaenoic acid
Biofuel production
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain yes
Preceptrol® no
Genome Sequenced Strain

Yes

Comments
ITS DNA sequencing results suggest that the strain likely has different rDNA operons, or the ITS sequence of this strain has unique features that make conventional DNA seqencing difficult.
Genome sequencing strain (the Joint Genome Institute at the Department of Energy, USA).
Medium ATCC® Medium 790: By+ medium
ATCC® Medium 633: M3 chytrid agar
ATCC® Medium 683: Koch's K-1 medium
Growth Conditions
Temperature: 18°C to 22°C
Atmosphere: Typical aerobic
Sequenced Data
Partial sequence of 18S ribosomal RNA gene, 5’ end

GTGTAAGTATAAGCGATTGTACTGTGAGACTGCGAACGGCTCATTATATCAGTAATAATTTCTTCGGTAGTTTCTTTTATATGGATACCTGCAGTAATTCTGGAAATAATACATGCTGTAAGAGCCCTGTATGGGGCTGCACTTATTAGATTGAAGCCGATTTTATTGGTGAATCATGATAATTGAGCAGATTGACTTTTTGGTCGATGAATCGTTTGAGTTTCTGCCCCATCAGTTGTCGACGGTAGTGTATTGGACTACGGTGACTATAACGGGTGACGGAGAGTTAGGGCTCGACTCCGGAGAGGGAGCCTGAGAGACGGCTACCATATCCAAGGATAGCAGCAGGCGCGTAAATTACCCACTGTGGACTCCACGAGGTAGTGACGAGAAATATCGATGCGAAGCGTGTATGCGTTTTGCTATCGGAATGAGAGCAATGTAAAACCCTCATC


Partial sequence of 18S ribosomal RNA gene, 3’ end

TGGAATTGAGTGCTTGGTCGGAAGGCCTGGCTAATCCTTGGAACGCTCATCGTGCTGGGGCTAGATTTTTGCAATTATTAATCTCCAACGAGGAATTCCTAGTAAACGCAAGTCATCAGCTTGCATTGAATACGTCCCTGCCCTTTGTACACACCGCCCGTCGCACCTACCGATTGAACGGTCCGATGAAACCATGGGATGTTTCTGTTTGGATTAATTTTTGGACAGAGGCAGAACTCGGGTGAATCTTATTGTTTAGAGGAAGG

Name of Depositor T Nakahara
Special Collection NCRR Contract
Chain of Custody
ATCC <-- T Nakahara <-- T Yokochi
Isolation
Seawater in mangrove area, Colonia, Yap Islands, Micronesia
Cross References

Nucleotide (GenBank) : AB022107 gene for 18S rRNA

References

Honda D, et al. Molecular phylogeny of labyrinthulids and thraustochytrids based on the sequencing of 18S ribosomal RNA gene. J. Eukaryot. Microbiol. 46: 637-647, 1999. PubMed: 10568038

Yokochi T, et al. Optimization of docosahexaenoic acid production by Schizochytrium limacinum SR21. Appl. Microbiol. Biotechnol. 49: 72-76, 1998.

Honda D, et al. Schizochytrium limacinum sp. nov., a new thraustochytrid from a mangrove area in the west Pacific Ocean. Mycol. Res. 102: 439-448, 1998.

Nakahara T, et al. Production of docosahexaenoic and docosapentaenoic acids by Schizochytrium sp. isolated from Yap Islands. J. Am. Oil Chem. Soc. 73: 1421-1426, 1996.

Ye C, et al. Reconstruction and analysis of the genome-scale metabolic model of Schizochytrium limacinum SR21 for docosahexaenoic acid production. BMC Genomics 16: 799, 2015. PubMed: 26475325

Liang Y, et al. Fermentable sugar release from Jatropha seed cakes following lime pretreatment and enzymatic hydrolysis. Bioresour Technol 101: 6417-6424, 2010. PubMed: 20417097

Liang Y, et al. Batch stage study of lipid production from crude glycerol derived from yellow grease or animal fats through microalgal fermentation. Bioresour Technol 101: 6745-6750, 2010. PubMed: 20381345

Liang Y, et al. Use of sweet sorghum juice for lipid production by Schizochytrium limacinum SR21. Bioresour Technol 101: 3623-3627, 2010. PubMed: 20079633

Chi Z, et al. Study of a two-stage growth of DHA-producing marine algae Schizochytrium limacinum SR21 with shifting dissolved oxygen level. Appl Microbiol Biotechnol 81: 1141-1148, 2009. PubMed: 18936938

Chi Z, et al. Production of omega-3 polyunsaturated fatty acids from cull potato using an algae culture process. Appl Biochem Biotechnol 137: 805-815, 2007. PubMed: 18478436

Miyamoto E, et al. Occurrence of 5'-deoxyadenosylcobalamin and its physiological function as the coenzyme of methylmalonyl-CoA mutase in a marine eukaryotic microorganism, Schizochytrium limacinum SR21. J Nutr Sci Vitaminol 53: 471-475, 2007. PubMed: 18202533

Morita E, et al. Docosahexaenoic acid production and lipid-body formation in Schizochytrium limacinum SR21. Mar Biotechnol 8: 319-327, 2006. PubMed: 16763939

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation