Crypthecodinium cohnii (Seligo) Javornicky (ATCC® 30775)

Organism: Crypthecodinium cohnii (Seligo) Javornicky  /  Depositor: CA Beam, M Himes

Permits and Restrictions

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Strain Designations SG-3
Application
Biofuel production
Biosafety Level 1
Isolation
Fucus sp., Sea Girt, Long Island, NY, 1977
Product Format frozen
Type Strain no
Comments
Minor Sibling Species SG
geographical distribution
sexuality and meiosis
Medium ATCC® Medium 460: A2E6 medium
Growth Conditions
Max Temperature: 25.0°C
Min Temperature: 20.0°C
Duration: axenic
Protocol: ATCCNO: 30021 SPEC: This culture is routinely shipped as a frozen stabilate. Thaw the ampule and aseptically transfer the material to 5 ml of ATCC medium 460 in a 16 x 125 mm screw-capped test tube. Do not distribute the thawed material to a larger volume of medium. It is essential to first establish the culture in a small volume. Screw cap on tightly, loosen one half turn, and incubate culture upright at 25C. The culture should be ready to subculture in approximately 7 days. To subculture, screw the cap on tightly, invert the culture 5 times and aseptically transfer a 0.1 ml aliquot to 5 ml of ATCC medium 460 and incubate as above. Prepare two subcultures weekly. Retain all cultures for up to one month to ensure against loss.
Subcultivation
Protocol: ATCCNO: 30021 SPEC: This culture is routinely shipped as a frozen stabilate. Thaw the ampule and aseptically transfer the material to 5 ml of ATCC medium 460 in a 16 x 125 mm screw-capped test tube. Do not distribute the thawed material to a larger volume of medium. It is essential to first establish the culture in a small volume. Screw cap on tightly, loosen one half turn, and incubate culture upright at 25C. The culture should be ready to subculture in approximately 7 days. To subculture, screw the cap on tightly, invert the culture 5 times and aseptically transfer a 0.1 ml aliquot to 5 ml of ATCC medium 460 and incubate as above. Prepare two subcultures weekly. Retain all cultures for up to one month to ensure against loss.
Name of Depositor CA Beam, M Himes
Year of Origin 1977
References

Beam CA, Himes M. Distribution of members of the Crypthecodinium cohnii (Dinophyceae) species complex. J. Protozool. 29: 8-15, 1982.

Beam CA, Himes M. Sexuality and meiosis in dinoflagellates. In: Beam CA, Himes M, Biochemistry and physiology of protozoa, 2nd ed.3 New York Academic Press: 171-206, 1980.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation