RDP 45/20 (ATCC® TIB-98)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with the MPC-11 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against the Igh-1b allotypic determinant on mouse IgG2a
Comments
Animals were immunized with CBPC-101 myeloma protein [IgG2a (Ig-1b)].
Spleen cells were fused with the MPC-11 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Isotype IgM
Name of Depositor WC Raschke
References

DiPauli R, Raschke WC. A hybridoma secreting IgM anti-IgIb allotype. Curr. Top. Microbiol. Immunol. 81: 37-39, 1978. PubMed: 567562

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

DiPauli R, Raschke WC. A hybridoma secreting IgM anti-IgIb allotype. Curr. Top. Microbiol. Immunol. 81: 37-39, 1978. PubMed: 567562