PAb 108 (ATCC® TIB-230)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody cross-reacts with the large T antigen of BK virus.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against a determinant on the N terminal region of large T antigen and small t antigen (SV40)
Cellular Products
immunoglobulin; monoclonal antibody; against a determinant on the N terminal region of large T antigen and small t antigen (SV40)
Comments
Animals were immunized with SV40 transformed BALB/3T3 cells.
Spleen cells were fused with NS-1 myeloma cells.
The antibody cross-reacts with the large T antigen of BK virus.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Dulbecco's modified Eagle's medium, 80%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG2a
Name of Depositor EG Gurney
References

Gurney EG, et al. Antigenic binding sites of monoclonal antibodies specific for simian virus 40 large T antigen. J. Virol. 57: 1168-1172, 1986. PubMed: 2419584

Basic Documentation
References

Gurney EG, et al. Antigenic binding sites of monoclonal antibodies specific for simian virus 40 large T antigen. J. Virol. 57: 1168-1172, 1986. PubMed: 2419584