PAb 108 (ATCC® TIB-230)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody cross-reacts with the large T antigen of BK virus.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against a determinant on the N terminal region of large T antigen and small t antigen (SV40)
Cellular Products
immunoglobulin; monoclonal antibody; against a determinant on the N terminal region of large T antigen and small t antigen (SV40)
Comments
Animals were immunized with SV40 transformed BALB/3T3 cells.
Spleen cells were fused with NS-1 myeloma cells.
The antibody cross-reacts with the large T antigen of BK virus.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Dulbecco's modified Eagle's medium, 80%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG2a
Name of Depositor EG Gurney
References

Gurney EG, et al. Antigenic binding sites of monoclonal antibodies specific for simian virus 40 large T antigen. J. Virol. 57: 1168-1172, 1986. PubMed: 2419584

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Gurney EG, et al. Antigenic binding sites of monoclonal antibodies specific for simian virus 40 large T antigen. J. Virol. 57: 1168-1172, 1986. PubMed: 2419584