LS-136 (ATCC® TIB-157)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody binds to the MOPC 104E, J 558 and HOPC 1 myeloma proteins but not to the MOPC 315 myeloma protein.
The antibody is specific for the C1 domain.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Animals were immunized with purified B1-8 monoclonal antibody.
Spleen cells were fused with P3X63Ag8 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse lambda 1 light chain
Cellular Products
immunoglobulin; monoclonal antibody; against mouse lambda 1 light chain
Comments
Animals were immunized with purified B1-8 monoclonal antibody.
Spleen cells were fused with P3X63Ag8 myeloma cells.
The antibody binds to the MOPC 104E, J 558 and HOPC 1 myeloma proteins but not to the MOPC 315 myeloma protein.
The antibody is specific for the C1 domain.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water and supplemented with 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Isotype IgG1; kappa light chain
Name of Depositor K Rajewsky
References

Reth M, et al. Analysis of the repertoire of anti-(4-hydroxy-3-nitro-phenyl) acetyl (NP) antibodies in C57 BL/6 mice by cell fusion. II. Characterization of idiotopes by monoclonal anti-idiotope antibodies. Eur. J. Immunol. 9: 1004-1013, 1979. PubMed: 398773

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Reth M, et al. Analysis of the repertoire of anti-(4-hydroxy-3-nitro-phenyl) acetyl (NP) antibodies in C57 BL/6 mice by cell fusion. II. Characterization of idiotopes by monoclonal anti-idiotope antibodies. Eur. J. Immunol. 9: 1004-1013, 1979. PubMed: 398773