alpha BL5C2.870016 [870016, aBL5C2.870016] (ATCC® HB-9909)

Organism: bovine (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

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Organism bovine (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody neutralizes BHV-1 and is protective against BHV-1 induced mortality in rabbits.
Derivation
Lymph node cells were fused with Sp2/0 mouse myeloma cells.
Initial hybrids were refused with additional lymph node cells from the same animal and reselected.
Genes Expressed
immunoglobulin; monoclonal antibody; against bovine herpesvirus 1 (BHV-1)
Cellular Products
immunoglobulin; monoclonal antibody; against bovine herpesvirus 1 (BHV-1)
Comments
Animals were immunized with live BHV-1.
Lymph node cells were fused with Sp2/0 mouse myeloma cells.
Initial hybrids were refused with additional lymph node cells from the same animal and reselected.
The antibody neutralizes BHV-1 and is protective against BHV-1 induced mortality in rabbits.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; horse serum, 10%
Complete Growth Medium
Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%;
horse serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
fetal bovine serum, 85%; DMSO, 15%
Isotype IgG1
Name of Depositor The United States of America
U.S. Patent Number
References

Levings RL, Stoll IR. Bovine monoclonal antibodies to bovine herpesvirus I from sequential fusion heterohybridomas. US Patent 5,026,646 dated Jun 25 1991

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Levings RL, Stoll IR. Bovine monoclonal antibodies to bovine herpesvirus I from sequential fusion heterohybridomas. US Patent 5,026,646 dated Jun 25 1991