EL-NC-1S [anti NAP-1] (ATCC® HB-9647)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

View Permits

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Strain

Strain: BALB/c (B cell); BALB/c (myeloma)

Applications
Animals were immunized with purified monocyte derived neutrophil chemotactic factor (MDNCF).
Derivation
Animals were immunized with purified monocyte derived neutrophil chemotactic factor (MDNCF).
Spleen cells were fused with NS-1 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against neutrophil attractant/activation protein 1 (NAP-1, also known as interleukin 8 or IL-8 and monocyte derived neutrophil chemotactic factor, MDNCF)
Cellular Products
immunoglobulin; monoclonal antibody; against neutrophil attractant/activation protein 1 (NAP-1, also known as interleukin 8 or IL-8 and monocyte derived neutrophil chemotactic factor, MDNCF)
Comments
Animals were immunized with purified monocyte derived neutrophil chemotactic factor (MDNCF).
Spleen cells were fused with NS-1 mouse myeloma cells.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG1
Name of Depositor National Cancer Institute/FCRF
References

Yoshimura T, et al. Three forms of monocyte-derived neutrophil chemotactic factor (MDNCF) distinguished by different lengths of the amino-terminal sequence. Mol. Immunol. 26: 87-93, 1989. PubMed: 2648135

Sylvester I, et al. Secretion of neutrophil attractant/activation protein by lipopolysaccharide-stimulated lung macrophages determined by both enzyme-linked immunosorbent assay and N-terminal sequence analysis. Am. Rev. Respir. Dis. 141: 683-688, 1990. PubMed: 2178529

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Yoshimura T, et al. Three forms of monocyte-derived neutrophil chemotactic factor (MDNCF) distinguished by different lengths of the amino-terminal sequence. Mol. Immunol. 26: 87-93, 1989. PubMed: 2648135

Sylvester I, et al. Secretion of neutrophil attractant/activation protein by lipopolysaccharide-stimulated lung macrophages determined by both enzyme-linked immunosorbent assay and N-terminal sequence analysis. Am. Rev. Respir. Dis. 141: 683-688, 1990. PubMed: 2178529