S6F1 (ATCC® HB-9579)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
Animals were immunized with the 1670 cell line produced by immortalizing spleen cells from a whitelip tamarin with Herpesvirus saimiri.
Derivation
Animals were immunized with the 1670 cell line produced by immortalizing spleen cells from a whitelip tamarin with Herpesvirus saimiri.
Spleen cells were fused with P3/NSI/1-Ag4-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against a subset of human CD8 (LFA-1) positive T cells
Cellular Products
immunoglobulin; monoclonal antibody; against a subset of human CD8 (LFA-1) positive T cells
Comments
Animals were immunized with the 1670 cell line produced by immortalizing spleen cells from a whitelip tamarin with Herpesvirus saimiri.
Spleen cells were fused with P3/NSI/1-Ag4-1 myeloma cells.
The antibody recognizes a subset of CD8 positive T cells that have cytotoxic effector activity.
The S6F1 epitope is absent on CD8 positive suppressor cells.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 2 mM L-glutamine, 0.1 mM hypoxanthine, and 0.032 mM thymidine, 80%; heat-inactivated fetal bovine serum, 10%; heat-inactivated horse serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor Dana Farber Cancer Inst.
References

Schlossman SF, Morimoto C. Monoclonal antibody specific to a novel epitope of the LFA-1 antigen of human T lymphocytes. US Patent 5,002,869 dated Mar 26 1991

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Schlossman SF, Morimoto C. Monoclonal antibody specific to a novel epitope of the LFA-1 antigen of human T lymphocytes. US Patent 5,002,869 dated Mar 26 1991