C180 [Clone 180] (ATCC® HB-9517)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Strain

Strain:  BALB/c (B cell); BALB/c (myeloma)

Applications
The antibody reacts preferentially with the a and b isoforms of KTI and binds equally well to KTI - trypsin complexes.
It reacts with native as opposed to denatured KTI.
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Kunitz soybean trypsin inhibitor
Cellular Products
immunoglobulin; monoclonal antibody; against Kunitz soybean trypsin inhibitor
Tumorigenic Yes
Effects
Yes, forms ascites in BALB/c mice
Comments
Animals were immunized with Kunitz soybean trypsin inhibitor (KTI).
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts preferentially with the a and b isoforms of KTI and binds equally well to KTI - trypsin complexes.
It reacts with native as opposed to denatured KTI.
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
  • Complete Growth Medium
    Culture medium: RPMI 1640 with 2 mM L-glutamine, 1 mM sodium pyruvate and
    10 mM HEPES, 85%; horse serum, 15%.
    Subculturing
    Medium Renewal: Every 2 to 3 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
    Cryopreservation
    culture medium 95%; DMSO, 5%
    Culture Conditions
    Temperature: 37.0°C
    Isotype IgG1; kappa light chain
    Name of Depositor The United States of America
    References

    Brandon DL, et al. Monoclonal antibodies to soybean Kunitz trypsin inhibitor and immunoassay methods. US Patent 4,959,310 dated Sep 25 1990

    Allison AC, et al. Monoclonal antibodies for interleukin-1.beta. US Patent 4,935,343 dated Jun 19 1990

    . . J. Food Sci. 53: 97-101, 1988.

    J. Agric. Food Chem. 36: 1336-1341, 1988.

    . . Crop Sci. 32: 1502-1505, 1992.

    J. Agric. Food Chem. 39: 327-335, 1991.

    . Nutritional and toxicologial consequences of food processing. New York: Plenum Press; 1991.

    . Protease inhibitors as cancer chemopreventive agents. New York: Plenum Press; 1993.

    Notice: Necessary PermitsPermits

    These permits may be required for shipping this product:

    • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
    Basic Documentation
    References

    Brandon DL, et al. Monoclonal antibodies to soybean Kunitz trypsin inhibitor and immunoassay methods. US Patent 4,959,310 dated Sep 25 1990

    Allison AC, et al. Monoclonal antibodies for interleukin-1.beta. US Patent 4,935,343 dated Jun 19 1990

    . . J. Food Sci. 53: 97-101, 1988.

    J. Agric. Food Chem. 36: 1336-1341, 1988.

    . . Crop Sci. 32: 1502-1505, 1992.

    J. Agric. Food Chem. 39: 327-335, 1991.

    . Nutritional and toxicologial consequences of food processing. New York: Plenum Press; 1991.

    . Protease inhibitors as cancer chemopreventive agents. New York: Plenum Press; 1993.