MECA-79 (ATCC® HB-9479)

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody specifically blocks lymphocyte adhesion to mouse peripheral lymph node endothelia and does not block lymphocyte binding to mouse Peyer's patches.
It binds to a sulfate and fucose dependent epitope associated with L-selectin binding ligands of high endothelial venules (HEV).
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse peripheral lymph node endothelial cells (peripheral node addressin)
Cellular Products
immunoglobulin; monoclonal antibody; against mouse peripheral lymph node endothelial cells (peripheral node addressin)
Comments
Animals were immunized with mouse lymph node stroma.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody specifically blocks lymphocyte adhesion to mouse peripheral lymph node endothelia and does not block lymphocyte binding to mouse Peyer's patches.
It binds to a sulfate and fucose dependent epitope associated with L-selectin binding ligands of high endothelial venules (HEV).
It inhibits L-selectin dependent lymphocyte homing to lymph nodes and blocks lymphocyte binding to HEV in vitro.
This line grows best when cultured with a feeder layer of mouse (BALB/c) peritoneal exudate cells.
Complete Growth Medium
RPMI 1640 medium with 2 mM L-glutamine adjusted to contain
1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium
pyruvate, 90%; fetal bovine serum, 10%; supplemented with 0.05 mM
2-mercaptoethanol. Note: A feeder layer of mouse peritoneal exudate or
irradiated murine fibroblasts (e.g. irradiated STO cells, ATCC catalog # X-56)
is required for recovery and growth of the MECA-79.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.
Culture Conditions
Temperature: 37.0°C
Isotype IgM
Name of Depositor Stanford University Board of Trustees
References

Butcher EC. Method to control leukocyte extravasation. US Patent 5,403,919 dated Apr 4 1995

Berg EL, et al. The human peripheral lymph node vascular addressin is a ligand for LECAM-1, the peripheral lymph node homing receptor. J. Cell Biol. 114: 343-349, 1991. PubMed: 1712790

Berg EL, et al. L-selectin-mediated lymphocyte rolling on MAdCAM-1. Nature 366: 695-698, 1993. PubMed: 7505053

Hemmerich S, et al. Sulfation-dependent recognition of high endothelial venules (HEV)- ligands by L-selectin and MECA 79, and adhesion-blocking monoclonal antibody. J. Exp. Med. 180: 2219-2226, 1994. PubMed: 7525849

Michie SA, et al. The human peripheral lymph node vascular addressin. An inducible endothelial antigen involved in lymphocyte homing. Am. J. Pathol. 143: 1688-1698, 1993. PubMed: 8256856

. . Cell. Biol. 107: 1853-1862, 1988.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Butcher EC. Method to control leukocyte extravasation. US Patent 5,403,919 dated Apr 4 1995

Berg EL, et al. The human peripheral lymph node vascular addressin is a ligand for LECAM-1, the peripheral lymph node homing receptor. J. Cell Biol. 114: 343-349, 1991. PubMed: 1712790

Berg EL, et al. L-selectin-mediated lymphocyte rolling on MAdCAM-1. Nature 366: 695-698, 1993. PubMed: 7505053

Hemmerich S, et al. Sulfation-dependent recognition of high endothelial venules (HEV)- ligands by L-selectin and MECA 79, and adhesion-blocking monoclonal antibody. J. Exp. Med. 180: 2219-2226, 1994. PubMed: 7525849

Michie SA, et al. The human peripheral lymph node vascular addressin. An inducible endothelial antigen involved in lymphocyte homing. Am. J. Pathol. 143: 1688-1698, 1993. PubMed: 8256856

. . Cell. Biol. 107: 1853-1862, 1988.